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1nt2

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[[Image:1nt2.jpg|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX==
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|PDB= 1nt2 |SIZE=350|CAPTION= <scene name='initialview01'>1nt2</scene>, resolution 2.90&Aring;
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<StructureSection load='1nt2' size='340' side='right'caption='[[1nt2]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
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<table><tr><td colspan='2'>[[1nt2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NT2 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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|GENE= AF2087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus]), AF2088 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nt2 OCA], [https://pdbe.org/1nt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nt2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nt2 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1fbn|1FBN]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nt2 OCA], [http://www.ebi.ac.uk/pdbsum/1nt2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nt2 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/FLPA_ARCFU FLPA_ARCFU] Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.<ref>PMID:15286083</ref>
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== Evolutionary Conservation ==
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'''CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1nt2_consurf.spt"</scriptWhenChecked>
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Nop56p and Nop58p are two core proteins of the box C/D snoRNPs that interact concurrently with fibrillarin and snoRNAs to function in enzyme assembly and catalysis. Here we report the 2.9 A resolution co-crystal structure of an archaeal homolog of Nop56p/Nop58p, Nop5p, in complex with fibrillarin from Archaeoglobus fulgidus (AF) and the methyl donor S-adenosyl-L-methionine. The N-terminal domain of Nop5p forms a complementary surface to fibrillarin that serves to anchor the catalytic subunit and to stabilize cofactor binding. A coiled coil in Nop5p mediates dimerization of two fibrillarin-Nop5p heterodimers for optimal interactions with bipartite box C/D RNAs. Structural analysis and complementary biochemical data demonstrate that the conserved C-terminal domain of Nop5p harbors RNA-binding sites. A model of box C/D snoRNP assembly is proposed based on the presented structural and biochemical data.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1NT2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT2 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nt2 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Structure and function of archaeal box C/D sRNP core proteins., Aittaleb M, Rashid R, Chen Q, Palmer JR, Daniels CJ, Li H, Nat Struct Biol. 2003 Apr;10(4):256-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12598892 12598892]
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<references/>
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[[Category: Archaeoglobus fulgidus]]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Aittaleb, M.]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Chen, Q.]]
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[[Category: Large Structures]]
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[[Category: Daniels, C J.]]
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[[Category: Aittaleb M]]
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[[Category: Li, H.]]
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[[Category: Chen Q]]
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[[Category: Palmer, J R.]]
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[[Category: Daniels CJ]]
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[[Category: Rashid, R.]]
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[[Category: Li H]]
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[[Category: ademet]]
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[[Category: Palmer JR]]
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[[Category: binding motif]]
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[[Category: Rashid R]]
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[[Category: coiled coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:35:05 2008''
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Current revision

CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX

PDB ID 1nt2

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