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| | ==Crystal structure of UndA with Iron Citrate bound== | | ==Crystal structure of UndA with Iron Citrate bound== |
| - | <StructureSection load='3ufh' size='340' side='right' caption='[[3ufh]], [[Resolution|resolution]] 2.23Å' scene=''> | + | <StructureSection load='3ufh' size='340' side='right'caption='[[3ufh]], [[Resolution|resolution]] 2.23Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ufh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shewanella_sp._hrcr-6 Shewanella sp. hrcr-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UFH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UFH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ufh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_sp._HRCR_06 Shewanella sp. HRCR_06]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UFH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ucp|3ucp]], [[3ufk|3ufk]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">undA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1043181 Shewanella sp. HRCR-6])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ufh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ufh OCA], [https://pdbe.org/3ufh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ufh RCSB], [https://www.ebi.ac.uk/pdbsum/3ufh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ufh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ufh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ufh OCA], [http://pdbe.org/3ufh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ufh RCSB], [http://www.ebi.ac.uk/pdbsum/3ufh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ufh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Shewanella sp. hrcr-6]] | + | [[Category: Large Structures]] |
| - | [[Category: Clarke, T A]] | + | [[Category: Shewanella sp. HRCR_06]] |
| - | [[Category: Edwards, M J]] | + | [[Category: Clarke TA]] |
| - | [[Category: C-type cytochrome]] | + | [[Category: Edwards MJ]] |
| - | [[Category: C-type heme]]
| + | |
| - | [[Category: Electron transfer]]
| + | |
| - | [[Category: Outermembrane]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
3ufh is a 1 chain structure with sequence from Shewanella sp. HRCR_06. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.23Å |
| Ligands: | , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Members of the genus Shewanella translocate deca- or undeca-heme cytochromes to the external cell surface thus enabling respiration using extracellular minerals and polynuclear Fe(III) chelates. The high resolution structure of the first undeca-heme outer membrane cytochrome, UndA, reveals a crossed heme chain with four potential electron ingress/egress sites arranged within four domains. Sequence and structural alignment of UndA and the deca-heme MtrF reveals the extra heme of UndA is inserted between MtrF hemes 6 and 7. The remaining UndA hemes can be superposed over the heme chain of the decaheme MtrF, suggesting that a ten heme core is conserved between outer membrane cytochromes. The UndA structure has also been crystallographically resolved in complex with substrates, an Fe(III)-nitrilotriacetate dimer or an Fe(III)-citrate trimer. The structural resolution of these UndA-Fe(III)-chelate complexes provides a rationale for previous kinetic measurements on UndA and other outer membrane cytochromes.
The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates.,Edwards MJ, Hall A, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA Structure. 2012 Jul 3;20(7):1275-84. Epub 2012 Jun 7. PMID:22682743[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Edwards MJ, Hall A, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA. The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates. Structure. 2012 Jul 3;20(7):1275-84. Epub 2012 Jun 7. PMID:22682743 doi:10.1016/j.str.2012.04.016
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