4f3m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:09, 1 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Crystal structure of CRISPR-associated protein==
==Crystal structure of CRISPR-associated protein==
-
<StructureSection load='4f3m' size='340' side='right' caption='[[4f3m]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
+
<StructureSection load='4f3m' size='340' side='right'caption='[[4f3m]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4f3m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F3M FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4f3m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_halodurans_C-125 Alkalihalobacillus halodurans C-125]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F3M FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BH0337 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272558 BACHD])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f3m OCA], [http://pdbe.org/4f3m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f3m RCSB], [http://www.ebi.ac.uk/pdbsum/4f3m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f3m ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f3m OCA], [https://pdbe.org/4f3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f3m RCSB], [https://www.ebi.ac.uk/pdbsum/4f3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f3m ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q9KFY3_ALKHC Q9KFY3_ALKHC] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).[PIRNR:PIRNR029950]
-
Clustered regularly interspaced short palindromic repeats (CRISPRs), together with an operon of CRISPR-associated (Cas) proteins, form an RNA-based prokaryotic immune system against exogenous genetic elements. Cas5 family proteins are found in several type I CRISPR-Cas systems. Here, we report the molecular function of subtype I-C/Dvulg Cas5d from Bacillus halodurans. We show that Cas5d cleaves pre-crRNA into unit length by recognizing both the hairpin structure and the 3' single stranded sequence in the CRISPR repeat region. Cas5d structure reveals a ferredoxin domain-based architecture and a catalytic triad formed by Y46, K116, and H117 residues. We further show that after pre-crRNA processing, Cas5d assembles with crRNA, Csd1, and Csd2 proteins to form a multi-sub-unit interference complex similar to Escherichia coli Cascade (CRISPR-associated complex for antiviral defense) in architecture. Our results suggest that formation of a crRNA-presenting Cascade-like complex is likely a common theme among type I CRISPR subtypes.
+
-
Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System.,Nam KH, Haitjema C, Liu X, Ding F, Wang H, Delisa MP, Ke A Structure. 2012 Jul 24. PMID:22841292<ref>PMID:22841292</ref>
+
==See Also==
-
 
+
*[[CRISPR subtype I-C|CRISPR subtype I-C]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4f3m" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bachd]]
+
[[Category: Alkalihalobacillus halodurans C-125]]
-
[[Category: Ke, A]]
+
[[Category: Large Structures]]
-
[[Category: Nam, K H]]
+
[[Category: Ke A]]
-
[[Category: Crispr]]
+
[[Category: Nam KH]]
-
[[Category: Endoribonuclease]]
+
-
[[Category: Ferredoxin fold]]
+
-
[[Category: Rna]]
+
-
[[Category: Rna binding protein]]
+

Current revision

Crystal structure of CRISPR-associated protein

PDB ID 4f3m

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4f3m"
Personal tools