This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3vnf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:40, 20 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Structure of the ebolavirus protein VP24 from Sudan==
==Structure of the ebolavirus protein VP24 from Sudan==
-
<StructureSection load='3vnf' size='340' side='right' caption='[[3vnf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+
<StructureSection load='3vnf' size='340' side='right'caption='[[3vnf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3vnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebolavirus_sudan Ebolavirus sudan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VNF FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3vnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sudan_ebolavirus Sudan ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VNF FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d9o|4d9o]], [[3vne|3vne]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186540 Ebolavirus Sudan])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vnf OCA], [https://pdbe.org/3vnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vnf RCSB], [https://www.ebi.ac.uk/pdbsum/3vnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vnf ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vnf OCA], [http://pdbe.org/3vnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vnf RCSB], [http://www.ebi.ac.uk/pdbsum/3vnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vnf ProSAT]</span></td></tr>
+
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/B0LPM0_9MONO B0LPM0_9MONO]] May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.[PIRNR:PIRNR011355]
+
[https://www.uniprot.org/uniprot/VP24_EBOSU VP24_EBOSU] Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1, by interacting with the STAT1-binding region of host importin alpha-1/KPNA1 protein, thereby inhibiting the latter. Without the activity of this protein, activated STAT1 would not enter the nucleus and be unable to activate IFN-induced genes. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Ebolaviruses cause hemorrhagic fever with up to 90% lethality and in fatal cases, are characterized by early suppression of the host innate immune system. One of the proteins likely responsible for this effect is VP24. VP24 is known to antagonize interferon signaling by binding host karyopherin alpha proteins, thereby preventing them from transporting the tyrosine-phosphorylated transcription factor STAT1 to the nucleus. Here, we report that VP24 binds STAT1 directly, suggesting that VP24 can suppress at least two distinct branches of the interferon pathway. Here, we also report the first crystal structures of VP24, derived from different species of ebolavirus that are pathogenic (Sudan) and nonpathogenic to humans (Reston). These structures reveal that VP24 has a novel, pyramidal fold. A site on a particular face of the pyramid exhibits reduced solvent exchange when in complex with STAT1. This site is above two highly conserved pockets in VP24 that contain key residues previously implicated in virulence. These crystal structures and accompanying biochemical analysis map differences between pathogenic and nonpathogenic viruses, offer templates for drug design, and provide the three-dimensional framework necessary for biological dissection of the many functions of VP24 in the virus life cycle.
+
-
 
+
-
The ebola virus interferon antagonist VP24 directly binds STAT1 and has a novel, pyramidal fold.,Zhang AP, Bornholdt ZA, Liu T, Abelson DM, Lee DE, Li S, Woods VL Jr, Saphire EO PLoS Pathog. 2012 Feb;8(2):e1002550. Epub 2012 Feb 23. PMID:22383882<ref>PMID:22383882</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3vnf" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
*[[VP24|VP24]]
*[[VP24|VP24]]
-
== References ==
 
-
<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Ebolavirus sudan]]
+
[[Category: Large Structures]]
-
[[Category: Zhang, A P.P]]
+
[[Category: Sudan ebolavirus]]
-
[[Category: Ebolavirus]]
+
[[Category: Zhang APP]]
-
[[Category: Ifn response pathway]]
+
-
[[Category: Interferon antagonist]]
+
-
[[Category: Karyopherin alpha]]
+
-
[[Category: Reston]]
+
-
[[Category: Stat1]]
+
-
[[Category: Sudan]]
+
-
[[Category: Viral protein]]
+
-
[[Category: Vp24]]
+
-
[[Category: Vp35]]
+
-
[[Category: Zaire]]
+

Current revision

Structure of the ebolavirus protein VP24 from Sudan

PDB ID 3vnf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vnf"
Personal tools