3va1

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==Crystal structure of the mammalian MDC1 FHA domain==
==Crystal structure of the mammalian MDC1 FHA domain==
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<StructureSection load='3va1' size='340' side='right' caption='[[3va1]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
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<StructureSection load='3va1' size='340' side='right'caption='[[3va1]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3va1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VA1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3va1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VA1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3va4|3va4]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mdc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3va1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3va1 OCA], [https://pdbe.org/3va1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3va1 RCSB], [https://www.ebi.ac.uk/pdbsum/3va1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3va1 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3va1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3va1 OCA], [http://pdbe.org/3va1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3va1 RCSB], [http://www.ebi.ac.uk/pdbsum/3va1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3va1 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MDC1_MOUSE MDC1_MOUSE]] Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (By similarity).
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[https://www.uniprot.org/uniprot/MDC1_MOUSE MDC1_MOUSE] Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mammalian MDC1 interacts with CHK2 in the regulation of DNA damage-induced S-phase checkpoint and apoptosis, which is directed by the association of MDC1-FHA and CHK2-pThr68. However, different ligand specificities of MDC1-FHA have been reported, and no structure is available. Here we report the crystal structures of MDC1-FHA and its complex with a CHK2 peptide containing pThr68. Unlike other FHA domains, MDC1-FHA exists as an intrinsic dimer in solution and in crystals. Structural and binding analyses support pThr+3 ligand specificity and provide structural insight into MDC1-CHK2 interaction.
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Structural Delineation of MDC1-FHA Domain Binding with CHK2-pThr68.,Wu HH, Wu PY, Huang KF, Kao YY, Tsai MD Biochemistry. 2012 Jan 17;51(2):575-7. Epub 2012 Jan 6. PMID:22211259<ref>PMID:22211259</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3va1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lk3 transgenic mice]]
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[[Category: Large Structures]]
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[[Category: Huang, K F]]
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[[Category: Mus musculus]]
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[[Category: Kao, Y Y]]
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[[Category: Huang KF]]
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[[Category: Tsai, M D]]
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[[Category: Kao YY]]
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[[Category: Wu, H H]]
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[[Category: Tsai MD]]
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[[Category: Wu, P Y]]
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[[Category: Wu HH]]
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[[Category: Cell cycle]]
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[[Category: Wu PY]]
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[[Category: Dna-damage]]
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[[Category: Fha domain]]
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[[Category: Mdc1 dimerization]]
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Current revision

Crystal structure of the mammalian MDC1 FHA domain

PDB ID 3va1

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