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| | ==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus== | | ==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus== |
| - | <StructureSection load='4n21' size='340' side='right' caption='[[4n21]], [[Resolution|resolution]] 1.99Å' scene=''> | + | <StructureSection load='4n21' size='340' side='right'caption='[[4n21]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n21]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Cas_virus Cas virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N21 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n21]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/CAS_virus CAS virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N21 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223561 CAS virus])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n21 OCA], [http://pdbe.org/4n21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n21 RCSB], [http://www.ebi.ac.uk/pdbsum/4n21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n21 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n21 OCA], [https://pdbe.org/4n21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n21 RCSB], [https://www.ebi.ac.uk/pdbsum/4n21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n21 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/J7H5L9_9VIRU J7H5L9_9VIRU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4n21" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4n21" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Glycoprotein GP 3D structures|Glycoprotein GP 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cas virus]] | + | [[Category: CAS virus]] |
| - | [[Category: Almo, S C]] | + | [[Category: Large Structures]] |
| - | [[Category: Dai, Z]] | + | [[Category: Almo SC]] |
| - | [[Category: Koellhoffer, J F]] | + | [[Category: Dai Z]] |
| - | [[Category: Lai, J R]] | + | [[Category: Koellhoffer JF]] |
| - | [[Category: Malashkevich, V N]] | + | [[Category: Lai JR]] |
| - | [[Category: Structural genomic]] | + | [[Category: Malashkevich VN]] |
| - | [[Category: Toro, R]] | + | [[Category: Toro R]] |
| - | [[Category: Nysgrc]]
| + | |
| - | [[Category: Post-fusion conformation]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
J7H5L9_9VIRU
Publication Abstract from PubMed
Fusion of the viral and host cell membranes is a necessary first step for infection by enveloped viruses and is mediated by the envelope glycoprotein. The transmembrane subunits from the structurally defined "class I" glycoproteins adopt an alpha-helical "trimer-of-hairpins" conformation during the fusion pathway. Here, we present our studies on the envelope glycoprotein transmembrane subunit, GP2, of the CAS virus (CASV). CASV was recently identified from annulated tree boas (Corallus annulatus) with inclusion body disease and is implicated in the disease etiology. We have generated and characterized two protein constructs consisting of the predicted CASV GP2 core domain. The crystal structure of the CASV GP2 post-fusion conformation indicates a trimeric alpha-helical bundle that is highly similar to those of Ebola virus and Marburg virus GP2 despite CASV genome homology to arenaviruses. Denaturation studies demonstrate that the stability of CASV GP2 is pH dependent with higher stability at lower pH; we propose that this behavior is due to a network of interactions among acidic residues that would destabilize the alpha-helical bundle under conditions where the side chains are deprotonated. The pH-dependent stability of the post-fusion structure has been observed in Ebola virus and Marburg virus GP2, as well as other viruses that enter via the endosome. Infection experiments with CASV and the related Golden Gate virus support a mechanism of entry that requires endosomal acidification. Our results suggest that, despite being primarily arenavirus like, the transmembrane subunit of CASV is extremely similar to the filoviruses.
Structural Characterization of the Glycoprotein GP2 Core Domain from the CAS Virus, a Novel Arenavirus-Like Species.,Koellhoffer JF, Dai Z, Malashkevich VN, Stenglein MD, Liu Y, Toro R, Harrison J, Chandran K, Derisi JL, Almo SC, Lai JR J Mol Biol. 2013 Dec 12. pii: S0022-2836(13)00751-1. doi:, 10.1016/j.jmb.2013.12.009. PMID:24333483[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Koellhoffer JF, Dai Z, Malashkevich VN, Stenglein MD, Liu Y, Toro R, Harrison J, Chandran K, Derisi JL, Almo SC, Lai JR. Structural Characterization of the Glycoprotein GP2 Core Domain from the CAS Virus, a Novel Arenavirus-Like Species. J Mol Biol. 2013 Dec 12. pii: S0022-2836(13)00751-1. doi:, 10.1016/j.jmb.2013.12.009. PMID:24333483 doi:http://dx.doi.org/10.1016/j.jmb.2013.12.009
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