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| | ==Ndc10: a platform for inner kinetochore assembly in budding yeast== | | ==Ndc10: a platform for inner kinetochore assembly in budding yeast== |
| - | <StructureSection load='3t79' size='340' side='right' caption='[[3t79]], [[Resolution|resolution]] 3.61Å' scene=''> | + | <StructureSection load='3t79' size='340' side='right'caption='[[3t79]], [[Resolution|resolution]] 3.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3t79]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T79 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3t79]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T79 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sqi|3sqi]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6113Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0E03807g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t79 OCA], [https://pdbe.org/3t79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t79 RCSB], [https://www.ebi.ac.uk/pdbsum/3t79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t79 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t79 OCA], [http://pdbe.org/3t79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t79 RCSB], [http://www.ebi.ac.uk/pdbsum/3t79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t79 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q6CPM4_KLULA Q6CPM4_KLULA] |
| - | Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)(2)-(Ndc10)(2) recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 A resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.
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| - | Ndc10 is a platform for inner kinetochore assembly in budding yeast.,Cho US, Harrison SC Nat Struct Mol Biol. 2011 Dec 4. doi: 10.1038/nsmb.2178. PMID:22139014<ref>PMID:22139014</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 3t79" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Candida sphaerica]] | + | [[Category: Kluyveromyces lactis NRRL Y-1140]] |
| - | [[Category: Cho, U S]] | + | [[Category: Large Structures]] |
| - | [[Category: Harrison, S C]] | + | [[Category: Cho US]] |
| - | [[Category: Dna]] | + | [[Category: Harrison SC]] |
| - | [[Category: Dna binding]]
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| - | [[Category: Dna binding protein-dna complex]]
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| - | [[Category: Dna recombinase]]
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