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| | ==Plasmodium falciparum SSB complex with ssDNA== | | ==Plasmodium falciparum SSB complex with ssDNA== |
| - | <StructureSection load='3ulp' size='340' side='right' caption='[[3ulp]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3ulp' size='340' side='right'caption='[[3ulp]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ulp]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ULP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ULP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ulp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ULP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ULP FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFE0435c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ulp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ulp OCA], [http://pdbe.org/3ulp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ulp RCSB], [http://www.ebi.ac.uk/pdbsum/3ulp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ulp ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ulp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ulp OCA], [https://pdbe.org/3ulp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ulp RCSB], [https://www.ebi.ac.uk/pdbsum/3ulp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ulp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8I415_PLAF7 Q8I415_PLAF7] |
| - | Single-stranded DNA binding (SSB) proteins play central roles in genome maintenance in all organisms. Plasmodium falciparum, the causative agent of malaria, encodes an SSB protein that localizes to the apicoplast and likely functions in the replication and maintenance of its genome. P. falciparum SSB (Pf-SSB) shares a high degree of sequence homology with bacterial SSB proteins but differs in the composition of its C-terminus, which interacts with more than a dozen other proteins in Escherichia coli SSB (Ec-SSB). Using sedimentation methods, we show that Pf-SSB forms a stable homo-tetramer alone and when bound to single-stranded DNA (ssDNA). We also present a crystal structure at 2.1 A resolution of the Pf-SSB tetramer bound to two (dT)(35) molecules. The Pf-SSB tetramer is structurally similar to the Ec-SSB tetramer, and ssDNA wraps completely around the tetramer with a "baseball seam" topology that is similar to Ec-SSB in its "65 binding mode". However, the polarity of the ssDNA wrapping around Pf-SSB is opposite to that observed for Ec-SSB. The interactions between the bases in the DNA and the amino acid side chains also differ from those observed in the Ec-SSB-DNA structure, suggesting that other differences may exist in the DNA binding properties of these structurally similar proteins.
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| - | Plasmodium falciparum SSB Tetramer Wraps Single-Stranded DNA with Similar Topology but Opposite Polarity to E. coli SSB.,Antony E, Weiland EA, Korolev S, Lohman TM J Mol Biol. 2012 Apr 27. PMID:22543099<ref>PMID:22543099</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3ulp" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plafa]] | + | [[Category: Large Structures]] |
| - | [[Category: Antony, E]] | + | [[Category: Plasmodium falciparum]] |
| - | [[Category: Korolev, S]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Lohman, T M]] | + | [[Category: Antony E]] |
| - | [[Category: Apicoplast]] | + | [[Category: Korolev S]] |
| - | [[Category: Dna binding]] | + | [[Category: Lohman TM]] |
| - | [[Category: Dna binding protein-dna complex]]
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| - | [[Category: Ob-fold]]
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| - | [[Category: Single-stranded dna]]
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