3tj1

<StructureSection load='3tj1' size='340' side='right' caption='[[3tj1]], [[Resolution|resolution]] 2.85&Aring;' scene=''>

<table><tr><td colspan='2'>[[3tj1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJ1 FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RRN3, YKL125W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tj1 OCA], [http://pdbe.org/3tj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tj1 RCSB], [http://www.ebi.ac.uk/pdbsum/3tj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tj1 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/RRN3_YEAST RRN3_YEAST]] Required for efficient transcription initiation by RNA polymerase I. Interacts with Pol I in the absence of template DNA and stimulates recruitment of Pol I, but does not remain as part of stable preinitiation complex.

Cell growth is regulated during RNA polymerase (Pol) I transcription initiation by the conserved factor Rrn3/TIF-IA in yeast/humans. Here we provide a structure-function analysis of Rrn3 based on a combination of structural biology with in vivo and in vitro functional assays. The Rrn3 crystal structure reveals a unique HEAT repeat fold and a surface serine patch. Phosphorylation of this patch represses human Pol I transcription, and a phospho-mimetic patch mutation prevents Rrn3 binding to Pol I in vitro and reduces cell growth and Pol I gene occupancy in vivo. Cross-linking indicates that Rrn3 binds Pol I between its subcomplexes, AC40/19 and A14/43, which faces the serine patch. The corresponding region of Pol II binds the Mediator head that cooperates with transcription factor (TF) IIB. Consistent with this, the Rrn3-binding factor Rrn7 is predicted to be a TFIIB homolog. This reveals the molecular basis of Rrn3-regulated Pol I initiation and cell growth, and indicates a general architecture of eukaryotic transcription initiation complexes.

 

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== References ==

[[Category: Baker's yeast]]

[[Category: Aebersold, R]]

[[Category: Blattner, C]]

[[Category: Cheung, A C.M]]

[[Category: Cramer, P]]

[[Category: Heck, A J.R]]

[[Category: Herzog, F]]

[[Category: Hopfner, K-P]]

[[Category: Jennebach, S]]

[[Category: Lorenzen, K]]

[[Category: Mayer, A]]

[[Category: Witte, G]]

[[Category: Transcription factor]]