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| | ==Tilted state of actin, T2== | | ==Tilted state of actin, T2== |
| - | <StructureSection load='3j8k' size='340' side='right' caption='[[3j8k]], [[Resolution|resolution]] 12.00Å' scene=''> | + | <SX load='3j8k' size='340' side='right' viewer='molstar' caption='[[3j8k]], [[Resolution|resolution]] 12.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3j8k]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J8K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3j8k]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J8K FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j8i|3j8i]], [[3j8j|3j8j]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8k OCA], [http://pdbe.org/3j8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j8k RCSB], [http://www.ebi.ac.uk/pdbsum/3j8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8k ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8k OCA], [https://pdbe.org/3j8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j8k RCSB], [https://www.ebi.ac.uk/pdbsum/3j8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8k ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | + | [https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Actin functions as a helical polymer, F-actin, but attempts to build an atomic model for this filament have been hampered by the fact that the filament cannot be crystallized and by structural heterogeneity. We have used a direct electron detector, cryo-electron microscopy, and the forces imposed on actin filaments in thin films to reconstruct one state of the filament at 4.7 A resolution, which allows for building a reliable pseudo-atomic model of F-actin. We also report a different state of the filament where actin protomers adopt a conformation observed in the crystal structure of the G-actin-profilin complex with an open ATP-binding cleft. Comparison of the two structural states provides insights into ATP-hydrolysis and filament dynamics. The atomic model provides a framework for understanding why every buried residue in actin has been under intense selective pressure.
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| - | Near-atomic resolution for one state of f-actin.,Galkin VE, Orlova A, Vos MR, Schroder GF, Egelman EH Structure. 2015 Jan 6;23(1):173-82. doi: 10.1016/j.str.2014.11.006. Epub 2014 Dec, 18. PMID:25533486<ref>PMID:25533486</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3j8k" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Actin|Actin]] | + | *[[Actin 3D structures|Actin 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | + | [[Category: Large Structures]] |
| | [[Category: Oryctolagus cuniculus]] | | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Egelman, E H]] | + | [[Category: Egelman EH]] |
| - | [[Category: Galkin, V E]] | + | [[Category: Galkin VE]] |
| - | [[Category: Orlova, A]] | + | [[Category: Orlova A]] |
| - | [[Category: Schroder, G F]] | + | [[Category: Schroder GF]] |
| - | [[Category: Vos, M R]] | + | [[Category: Vos MR]] |
| - | [[Category: Actin filament]]
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| - | [[Category: Helical polymer]]
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| - | [[Category: Structural protein]]
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