4gk8

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==Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and L-histidinol arsenate==
==Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and L-histidinol arsenate==
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<StructureSection load='4gk8' size='340' side='right' caption='[[4gk8]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
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<StructureSection load='4gk8' size='340' side='right'caption='[[4gk8]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GK8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GK8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GK8:{[(2S)-2-AMINO-3-(1H-IMIDAZOL-5-YL)PROPYL]OXY}(TRIHYDROXY)-LAMBDA~5~-ARSANYL'>GK8</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.933&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gc3|4gc3]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GK8:{[(2S)-2-AMINO-3-(1H-IMIDAZOL-5-YL)PROPYL]OXY}(TRIHYDROXY)-LAMBDA~5~-ARSANYL'>GK8</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hisK, LL1216, L37351 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gk8 OCA], [https://pdbe.org/4gk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gk8 RCSB], [https://www.ebi.ac.uk/pdbsum/4gk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gk8 ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gk8 OCA], [http://pdbe.org/4gk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gk8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gk8 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/HIS9_LACLA HIS9_LACLA]
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L-Histidinol phosphate phosphatase (HPP) catalyzes the hydrolysis of L-histidinol phosphate to L-histidinol and inorganic phosphate, the penultimate step in the biosynthesis of L-histidine. HPP from the polymerase and histidinol phosphatase (PHP) family of proteins possesses a trinuclear active site and a distorted (beta/alpha)7-barrel protein fold. This group of enzymes is closely related to the amidohydrolase superfamily of enzymes. The mechanism of phosphomonoester bond hydrolysis by the PHP family of HPP enzymes was addressed. Recombinant HPP from Lactococcus lactis subsp. lactis that was expressed in Escherichia coli contained a mixture of iron and zinc in the active site and had a catalytic efficiency of ~103 M-1 s-1. Expression of the protein under iron-free conditions resulted in the production of enzyme with a two orders of magnitude improvement in catalytic efficiency and a mixture of zinc and manganese in the active site. Solvent isotope and viscosity effects demonstrated that proton transfer steps and product dissociation steps are not rate-limiting. X-ray structures of HPP were determined with sulfate, L-histidinol/phosphate, and a complex of L-histidinol and arsenate bound in the active site. These crystal structures and the catalytic properties of site-directed mutants were used to identify the structural elements required for catalysis and substrate recognition by the HPP family of enzymes within the amidohydrolase superfamily.
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Structural and Mechanistic Characterization of L-Histidinol Phosphate Phosphatase from the PHP Family of Proteins.,Ghodge SV, Fedorov AA, Fedorov EV, Hillerich B, Seidel RD, Almo SC, Raushel FM Biochemistry. 2013 Jan 17. PMID:23327428<ref>PMID:23327428</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4gk8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Histidinol-phosphatase]]
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[[Category: Lactococcus lactis subsp. lactis Il1403]]
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[[Category: Lacla]]
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[[Category: Large Structures]]
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[[Category: Almo, S C]]
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[[Category: Almo SC]]
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[[Category: Fedorov, A A]]
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[[Category: Fedorov AA]]
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[[Category: Fedorov, E V]]
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[[Category: Fedorov EV]]
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[[Category: Ghodge, S]]
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[[Category: Ghodge S]]
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[[Category: Raushel, F M]]
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[[Category: Raushel FM]]
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[[Category: Hydrolase]]
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[[Category: Php fold]]
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Current revision

Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and L-histidinol arsenate

PDB ID 4gk8

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