|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of N-terminal first spectrin repeat of utrophin== | | ==Crystal Structure of N-terminal first spectrin repeat of utrophin== |
| - | <StructureSection load='3uum' size='340' side='right' caption='[[3uum]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3uum' size='340' side='right'caption='[[3uum]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UUM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UUM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3uul|3uul]], [[3uun|3uun]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Utrn ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uum OCA], [https://pdbe.org/3uum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uum RCSB], [https://www.ebi.ac.uk/pdbsum/3uum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uum ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uum OCA], [http://pdbe.org/3uum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uum RCSB], [http://www.ebi.ac.uk/pdbsum/3uum PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uum ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/O55147_RAT O55147_RAT] |
| - | Dystrophin and utrophin link the F-actin cytoskeleton to the cell membrane via an associated glycoprotein complex. This functionality results from their domain organization having an N-terminal actin-binding domain followed by multiple spectrin-repeat domains and then C-terminal protein-binding motifs. Therapeutic strategies to replace defective dystrophin with utrophin in patients with Duchenne muscular dystrophy require full-characterization of both these proteins to assess their degree of structural and functional equivalence. Here the high resolution structures of the first spectrin repeats (N-terminal repeat 1) from both dystrophin and utrophin have been determined by x-ray crystallography. The repeat structures both display a three-helix bundle fold very similar to one another and to homologous domains from spectrin, alpha-actinin and plectin. The utrophin and dystrophin repeat structures reveal the relationship between the structural domain and the canonical spectrin repeat domain sequence motif, showing the compact structural domain of spectrin repeat one to be extended at the C-terminus relative to its previously defined sequence repeat. These structures explain previous in vitro biochemical studies in which extending dystrophin spectrin repeat domain length leads to increased protein stability. Furthermore we show that the first dystrophin and utrophin spectrin repeats have no affinity for F-actin in the absence of other domains.
| + | |
| - | | + | |
| - | The crystal structures of dystrophin and utrophin spectrin repeats: implications for domain boundaries.,Muthu M, Richardson KA, Sutherland-Smith AJ PLoS One. 2012;7(7):e40066. Epub 2012 Jul 20. PMID:22911693<ref>PMID:22911693</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3uum" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Large Structures]] |
| - | [[Category: Muthu, M]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Richardson, K A]] | + | [[Category: Muthu M]] |
| - | [[Category: Sutherland-smith, A J]] | + | [[Category: Richardson KA]] |
| - | [[Category: Cytoskeletal]] | + | [[Category: Sutherland-smith AJ]] |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Structural stability]]
| + | |
| - | [[Category: Triple helical]]
| + | |
