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| | ==Apo HA17-HA33== | | ==Apo HA17-HA33== |
| - | <StructureSection load='4lo0' size='340' side='right' caption='[[4lo0]], [[Resolution|resolution]] 2.06Å' scene=''> | + | <StructureSection load='4lo0' size='340' side='right'caption='[[4lo0]], [[Resolution|resolution]] 2.06Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lo0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LO0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lo0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LO0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lo1|4lo1]], [[4lo2|4lo2]], [[4lo3|4lo3]], [[4lo4|4lo4]], [[4lo5|4lo5]], [[4lo6|4lo6]], [[4lo7|4lo7]], [[4lo8|4lo8]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.055Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HA-33, ha33, ha34 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896]), ha17, HA-17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lo0 OCA], [https://pdbe.org/4lo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lo0 RCSB], [https://www.ebi.ac.uk/pdbsum/4lo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lo0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lo0 OCA], [http://pdbe.org/4lo0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lo0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lo0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lo0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q45871_CLOBO Q45871_CLOBO] |
| - | Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary proteins as progenitor toxin complexes (PTCs) to become highly potent oral poisons. Here, we report the structure of a approximately 760 kDa 14-subunit large PTC of serotype A (L-PTC/A) and reveal insight into its absorption mechanism. Using a combination of X-ray crystallography, electron microscopy, and functional studies, we found that L-PTC/A consists of two structurally and functionally independent sub-complexes. A hetero-dimeric 290 kDa complex protects BoNT, while a hetero-dodecameric 470 kDa complex facilitates its absorption in the harsh environment of the gastrointestinal tract. BoNT absorption is mediated by nine glycan-binding sites on the dodecameric sub-complex that forms multivalent interactions with carbohydrate receptors on intestinal epithelial cells. We identified monosaccharides that blocked oral BoNT intoxication in mice, which suggests a new strategy for the development of preventive countermeasures for BoNTs based on carbohydrate receptor mimicry.
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| - | Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.,Lee K, Gu S, Jin L, Le TT, Cheng LW, Strotmeier J, Kruel AM, Yao G, Perry K, Rummel A, Jin R PLoS Pathog. 2013 Oct;9(10):e1003690. doi: 10.1371/journal.ppat.1003690. Epub, 2013 Oct 10. PMID:24130488<ref>PMID:24130488</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4lo0" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus botulinus van ermengem 1896]] | + | [[Category: Clostridium botulinum]] |
| - | [[Category: Cheng, L W]] | + | [[Category: Large Structures]] |
| - | [[Category: Gu, S]] | + | [[Category: Cheng LW]] |
| - | [[Category: Jin, L]] | + | [[Category: Gu S]] |
| - | [[Category: Jin, R]] | + | [[Category: Jin L]] |
| - | [[Category: Kruel, A M]] | + | [[Category: Jin R]] |
| - | [[Category: Le, T T]] | + | [[Category: Kruel AM]] |
| - | [[Category: Lee, K]] | + | [[Category: Le TT]] |
| - | [[Category: Perry, K]] | + | [[Category: Lee K]] |
| - | [[Category: Rummel, A]] | + | [[Category: Perry K]] |
| - | [[Category: Strotmeier, J]] | + | [[Category: Rummel A]] |
| - | [[Category: Yao, G]] | + | [[Category: Strotmeier J]] |
| - | [[Category: Botulinum neurotoxin]]
| + | [[Category: Yao G]] |
| - | [[Category: Botulism]]
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| - | [[Category: Carbohydrate/sugar binding]]
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| - | [[Category: Hemagglutinin]]
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| - | [[Category: Neurotoxin associated protein]]
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| - | [[Category: Progenitor toxin complex]]
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| - | [[Category: Protein transport]]
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| - | [[Category: Secreted protein]]
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