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4jhj

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Crystal structure of Danio rerio Slip1 in complex with Dbp5

Structural highlights

4jhj is a 4 chain structure with sequence from Danio rerio. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

M4GDB_DANRE Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically enhancing their translation (By similarity).

Publication Abstract from PubMed

In metazoans, replication-dependent histone mRNAs end in a stem-loop structure instead of the poly(A) tail characteristic of all other mature mRNAs. This specialized 3' end is bound by stem-loop binding protein (SLBP), a protein that participates in the nuclear export and translation of histone mRNAs. The translational activity of SLBP is mediated by interaction with SLIP1, a middle domain of initiation factor 4G (MIF4G)-like protein that connects to translation initiation. We determined the 2.5 A resolution crystal structure of zebrafish SLIP1 bound to the translation-activation domain of SLBP and identified the determinants of the recognition. We discovered a SLIP1-binding motif (SBM) in two additional proteins: the translation initiation factor eIF3g and the mRNA-export factor DBP5. We confirmed the binding of SLIP1 to DBP5 and eIF3g by pull-down assays and determined the 3.25 A resolution structure of SLIP1 bound to the DBP5 SBM. The SBM-binding and homodimerization residues of SLIP1 are conserved in the MIF4G domain of CBP80/20-dependent translation initiation factor (CTIF). The results suggest how the SLIP1 homodimer or a SLIP1-CTIF heterodimer can function as platforms to bridge SLBP with SBM-containing proteins involved in different steps of mRNA metabolism.

Structural and biochemical studies of SLIP1-SLBP identify DBP5 and eIF3g as SLIP1-binding proteins.,von Moeller H, Lerner R, Ricciardi A, Basquin C, Marzluff WF, Conti E Nucleic Acids Res. 2013 Jun 26. PMID:23804756^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ von Moeller H, Lerner R, Ricciardi A, Basquin C, Marzluff WF, Conti E. Structural and biochemical studies of SLIP1-SLBP identify DBP5 and eIF3g as SLIP1-binding proteins. Nucleic Acids Res. 2013 Jun 26. PMID:23804756 doi:10.1093/nar/gkt558

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4jhj"

Categories: Danio rerio | Large Structures | Conti E | Von Moeller H

@@ Line 1: / Line 1: @@
 ==Crystal structure of Danio rerio Slip1 in complex with Dbp5==
-<StructureSection load='4jhj' size='340' side='right' caption='[[4jhj]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
+<StructureSection load='4jhj' size='340' side='right'caption='[[4jhj]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4jhj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JHJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4jhj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JHJ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3fht|3fht]], [[3fhc|3fhc]], [[2i2o|2i2o]], [[4jhk|4jhk]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mif4gdb, zgc:110826 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio]), ddx19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jhj OCA], [https://pdbe.org/4jhj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jhj RCSB], [https://www.ebi.ac.uk/pdbsum/4jhj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jhj ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jhj OCA], [http://pdbe.org/4jhj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jhj RCSB], [http://www.ebi.ac.uk/pdbsum/4jhj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jhj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/M4GDB_DANRE M4GDB_DANRE]] Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically enhancing their translation (By similarity).
+[https://www.uniprot.org/uniprot/M4GDB_DANRE M4GDB_DANRE] Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically enhancing their translation (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Brachidanio rerio]]
+[[Category: Danio rerio]]
-[[Category: Conti, E]]
+[[Category: Large Structures]]
-[[Category: Moeller, H Von]]
+[[Category: Conti E]]
-[[Category: 3'-processing]]
+[[Category: Von Moeller H]]
-[[Category: 3'-utr]]
-[[Category: Atp binding]]
-[[Category: Ddx19]]
-[[Category: Dead-box helicase]]
-[[Category: Dead-box protein 19b]]
-[[Category: Helicase]]
-[[Category: Hydrolase]]
-[[Category: Mrna export]]
-[[Category: Mrna transport]]
-[[Category: Nucleotide binding]]
-[[Category: Nup214]]
-[[Category: Reca-like]]
-[[Category: Rna binding]]
-[[Category: Rna-dependent atpase]]
-[[Category: Translation]]
-[[Category: Transport histone mrna processing]]

4jhj

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Current revision

Crystal structure of Danio rerio Slip1 in complex with Dbp5

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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