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| | ==E. coli O157 ParE2-associated antitoxin 2 (PaaA2)== | | ==E. coli O157 ParE2-associated antitoxin 2 (PaaA2)== |
| - | <StructureSection load='3zbe' size='340' side='right' caption='[[3zbe]], [[NMR_Ensembles_of_Models | 50 NMR models]]' scene=''> | + | <StructureSection load='3zbe' size='340' side='right'caption='[[3zbe]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zbe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZBE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zbe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZBE FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zbe OCA], [http://pdbe.org/3zbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zbe RCSB], [http://www.ebi.ac.uk/pdbsum/3zbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zbe ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zbe OCA], [https://pdbe.org/3zbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zbe RCSB], [https://www.ebi.ac.uk/pdbsum/3zbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zbe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8XAD5_ECO57 Q8XAD5_ECO57] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eco57]] | + | [[Category: Escherichia coli O157:H7]] |
| - | [[Category: Loris, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Nuland, N A.J Van]] | + | [[Category: Loris R]] |
| - | [[Category: Sterckx, Y G.J]] | + | [[Category: Sterckx YGJ]] |
| - | [[Category: Vranken, W F]] | + | [[Category: Van Nuland NAJ]] |
| - | [[Category: Partially disordered protein]] | + | [[Category: Vranken WF]] |
| - | [[Category: Saxs/nmr]]
| + | |
| - | [[Category: Toxin-antitoxin]]
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| Structural highlights
Function
Q8XAD5_ECO57
Publication Abstract from PubMed
Antitoxins from prokaryotic type II toxin-antitoxin modules are characterized by a high degree of intrinsic disorder. The description of such highly flexible proteins is challenging because they cannot be represented by a single structure. Here, we present a combination of SAXS and NMR data to describe the conformational ensemble of the PaaA2 antitoxin from the human pathogen E. coli O157. The method encompasses the use of SAXS data to filter ensembles out of a pool of conformers generated by a custom NMR structure calculation protocol and the subsequent refinement by a block jackknife procedure. The final ensemble obtained through the method is validated by an established residual dipolar coupling analysis. We show that the conformational ensemble of PaaA2 is highly compact and that the protein exists in solution as two preformed helices, connected by a flexible linker, that probably act as molecular recognition elements for toxin inhibition.
Small-Angle X-Ray Scattering- and Nuclear Magnetic Resonance-Derived Conformational Ensemble of the Highly Flexible Antitoxin PaaA2.,Sterckx YG, Volkov AN, Vranken WF, Kragelj J, Jensen MR, Buts L, Garcia-Pino A, Jove T, Van Melderen L, Blackledge M, van Nuland NA, Loris R Structure. 2014 Apr 23. pii: S0969-2126(14)00084-7. doi:, 10.1016/j.str.2014.03.012. PMID:24768114[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sterckx YG, Volkov AN, Vranken WF, Kragelj J, Jensen MR, Buts L, Garcia-Pino A, Jove T, Van Melderen L, Blackledge M, van Nuland NA, Loris R. Small-Angle X-Ray Scattering- and Nuclear Magnetic Resonance-Derived Conformational Ensemble of the Highly Flexible Antitoxin PaaA2. Structure. 2014 Apr 23. pii: S0969-2126(14)00084-7. doi:, 10.1016/j.str.2014.03.012. PMID:24768114 doi:http://dx.doi.org/10.1016/j.str.2014.03.012
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