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| | ==Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member== | | ==Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member== |
| - | <StructureSection load='4kji' size='340' side='right' caption='[[4kji]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='4kji' size='340' side='right'caption='[[4kji]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4kji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseab Pseab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KJI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4kji]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KJI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA14_68470, rsmN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kji OCA], [http://pdbe.org/4kji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kji RCSB], [http://www.ebi.ac.uk/pdbsum/4kji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kji ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kji OCA], [https://pdbe.org/4kji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kji RCSB], [https://www.ebi.ac.uk/pdbsum/4kji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kji ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H2ZIZ8_PSEAB A0A0H2ZIZ8_PSEAB] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseab]] | + | [[Category: Large Structures]] |
| - | [[Category: Li, C]] | + | [[Category: Pseudomonas aeruginosa UCBPP-PA14]] |
| - | [[Category: Beta barrel]]
| + | [[Category: Li C]] |
| - | [[Category: Beta-barrel]]
| + | |
| - | [[Category: Post-transcriptional regulation]] | + | |
| - | [[Category: Protein-rna complex]]
| + | |
| - | [[Category: Rna binding]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| Structural highlights
Function
A0A0H2ZIZ8_PSEAB
Publication Abstract from PubMed
In bacteria, the highly conserved RsmA/CsrA family of RNA-binding proteins functions as global posttranscriptional regulators acting on mRNA translation and stability. Through phenotypic complementation of an rsmA mutant in Pseudomonas aeruginosa, we discovered a family member, termed RsmN. Elucidation of the RsmN crystal structure and that of the complex with a hairpin from the sRNA, RsmZ, reveals a uniquely inserted alpha helix, which redirects the polypeptide chain to form a distinctly different protein fold to the domain-swapped dimeric structure of RsmA homologs. The overall beta sheet structure required for RNA recognition is, however, preserved with compensatory sequence and structure differences, allowing the RsmN dimer to target binding motifs in both structured hairpin loops and flexible disordered RNAs. Phylogenetic analysis indicates that, although RsmN appears unique to P. aeruginosa, homologous proteins with the inserted alpha helix are more widespread and arose as a consequence of a gene duplication event.
Structural Rearrangement in an RsmA/CsrA Ortholog of Pseudomonas aeruginosa Creates a Dimeric RNA-Binding Protein, RsmN.,Morris ER, Hall G, Li C, Heeb S, Kulkarni RV, Lovelock L, Silistre H, Messina M, Camara M, Emsley J, Williams P, Searle MS Structure. 2013 Aug 13. pii: S0969-2126(13)00257-8. doi:, 10.1016/j.str.2013.07.007. PMID:23954502[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morris ER, Hall G, Li C, Heeb S, Kulkarni RV, Lovelock L, Silistre H, Messina M, Camara M, Emsley J, Williams P, Searle MS. Structural Rearrangement in an RsmA/CsrA Ortholog of Pseudomonas aeruginosa Creates a Dimeric RNA-Binding Protein, RsmN. Structure. 2013 Aug 13. pii: S0969-2126(13)00257-8. doi:, 10.1016/j.str.2013.07.007. PMID:23954502 doi:10.1016/j.str.2013.07.007
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