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| - | ==GELSOLIN NANOBODY== | + | ==Gelsolin Nanobody== |
| - | <StructureSection load='2x1p' size='340' side='right' caption='[[2x1p]], [[Resolution|resolution]] 1.10Å' scene=''> | + | <StructureSection load='2x1p' size='340' side='right'caption='[[2x1p]], [[Resolution|resolution]] 1.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2x1p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X1P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2x1p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X1P FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x1q|2x1q]], [[2x1o|2x1o]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x1p OCA], [http://pdbe.org/2x1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x1p RCSB], [http://www.ebi.ac.uk/pdbsum/2x1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x1p ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x1p OCA], [https://pdbe.org/2x1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x1p RCSB], [https://www.ebi.ac.uk/pdbsum/2x1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x1p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[Gelsolin|Gelsolin]] | + | *[[Gelsolin 3D structures|Gelsolin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Camelus glama]] | + | [[Category: Lama glama]] |
| - | [[Category: Abbeele, A Van Den]] | + | [[Category: Large Structures]] |
| - | [[Category: Corte, V De]] | + | [[Category: De Corte V]] |
| - | [[Category: Declercq, S]] | + | [[Category: De Ganck A]] |
| - | [[Category: Ganck, A De]] | + | [[Category: Declercq S]] |
| - | [[Category: Gettemans, J]] | + | [[Category: Gettemans J]] |
| - | [[Category: Kerckhove, J Van De]] | + | [[Category: Srinivasan V]] |
| - | [[Category: Loo, B Van]] | + | [[Category: Steyaert J]] |
| - | [[Category: Srinivasan, V]] | + | [[Category: Van De Kerckhove J]] |
| - | [[Category: Steyaert, J]] | + | [[Category: Van Den Abbeele A]] |
| - | [[Category: Contractile protein]] | + | [[Category: Van Loo B]] |
| Structural highlights
Publication Abstract from PubMed
RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated.
A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.,Van den Abbeele A, De Clercq S, De Ganck A, De Corte V, Van Loo B, Soror SH, Srinivasan V, Steyaert J, Vandekerckhove J, Gettemans J Cell Mol Life Sci. 2010 May;67(9):1519-35. Epub 2010 Feb 7. PMID:20140750[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Van den Abbeele A, De Clercq S, De Ganck A, De Corte V, Van Loo B, Soror SH, Srinivasan V, Steyaert J, Vandekerckhove J, Gettemans J. A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction. Cell Mol Life Sci. 2010 May;67(9):1519-35. Epub 2010 Feb 7. PMID:20140750 doi:10.1007/s00018-010-0266-1
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