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| | ==GrpN pentameric microcompartment shell protein from Rhodospirillum rubrum== | | ==GrpN pentameric microcompartment shell protein from Rhodospirillum rubrum== |
| - | <StructureSection load='4i7a' size='340' side='right' caption='[[4i7a]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='4i7a' size='340' side='right'caption='[[4i7a]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4i7a]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum_f11 Rhodospirillum rubrum f11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I7A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4i7a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillum_rubrum_F11 Rhodospirillum rubrum F11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I7A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F11_04705, grpN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1036743 Rhodospirillum rubrum F11])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i7a OCA], [http://pdbe.org/4i7a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i7a RCSB], [http://www.ebi.ac.uk/pdbsum/4i7a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i7a ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i7a OCA], [https://pdbe.org/4i7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i7a RCSB], [https://www.ebi.ac.uk/pdbsum/4i7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i7a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Rhodospirillum rubrum f11]] | + | [[Category: Large Structures]] |
| - | [[Category: Cascio, D]] | + | [[Category: Rhodospirillum rubrum F11]] |
| - | [[Category: Gidaniyan, S D]] | + | [[Category: Cascio D]] |
| - | [[Category: Wheatley, N M]] | + | [[Category: Gidaniyan SD]] |
| - | [[Category: Yeates, T O]] | + | [[Category: Wheatley NM]] |
| - | [[Category: Ccml]] | + | [[Category: Yeates TO]] |
| - | [[Category: Eutn]]
| + | |
| - | [[Category: Glycyl radical propanediol microcompartment]]
| + | |
| - | [[Category: Microcompartment]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Bacterial microcompartments (MCPs) are large proteinaceous structures comprised of a roughly icosahedral shell and a series of encapsulated enzymes. MCPs carrying out three different metabolic functions have been characterized in some detail, while gene expression and bioinformatics studies have implicated other types, including one believed to perform glycyl radical-based metabolism of 1,2-propanediol (Grp). Here we report the crystal structure of a protein (GrpN), which is presumed to be part of the shell of a Grp-type MCP in Rhodospirillum rubrum F11. GrpN is homologous to a family of proteins (EutN/PduN/CcmL/CsoS4) whose members have been implicated in forming the vertices of MCP shells. Consistent with that notion, the crystal structure of GrpN revealed a pentameric assembly. That observation revived an outstanding question about the oligomeric state of this protein family: pentameric forms (for CcmL and CsoS4A) and a hexameric form (for EutN) had both been observed in previous crystal structures. To clarify these confounding observations, we revisited the case of EutN. We developed a molecular biology-based method for accurately determining the number of subunits in homo-oligomeric proteins, and found unequivocally that EutN is a pentamer in solution. Based on these convergent findings, we propose the name bacterial microcompartment vertex for this special family of MCP shell proteins.
Bacterial microcompartment shells of diverse functional types possess pentameric vertex proteins.,Wheatley NM, Gidaniyan SD, Liu Y, Cascio D, Yeates TO Protein Sci. 2013 May;22(5):660-5. doi: 10.1002/pro.2246. Epub 2013 Apr 8. PMID:23456886[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wheatley NM, Gidaniyan SD, Liu Y, Cascio D, Yeates TO. Bacterial microcompartment shells of diverse functional types possess pentameric vertex proteins. Protein Sci. 2013 May;22(5):660-5. doi: 10.1002/pro.2246. Epub 2013 Apr 8. PMID:23456886 doi:http://dx.doi.org/10.1002/pro.2246
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