4fnj

<StructureSection load='4fnj' size='340' side='right' caption='[[4fnj]], [[Resolution|resolution]] 1.95&Aring;' scene=''>

<table><tr><td colspan='2'>[[4fnj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FNJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FNJ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fnj OCA], [http://pdbe.org/4fnj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fnj RCSB], [http://www.ebi.ac.uk/pdbsum/4fnj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fnj ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Myotonic dystrophy type 1 (DM1) is a microsatellite expansion disorder caused by the aberrant expansion of CTG repeats in the 3'-untranslated region of the DMPK gene. When transcribed, the toxic RNA CUG repeats sequester RNA binding proteins, which leads to disease symptoms. The expanded CUG repeats can adopt a double-stranded structure, and targeting this helix is a therapeutic strategy for DM1. To improve our understanding of the 5'CUG/3'GUC motif and how it may interact with proteins and small molecules, we designed a short CUG helix attached to a GAAA tetraloop/receptor to facilitate crystal packing. Here we report the highest-resolution structure (1.95 A) to date of a GAAA tetraloop/receptor and the CUG helix it was used to crystallize. Within the CUG helix, we identify two different forms of noncanonical U-U pairs and reconfirm that CUG repeats are essentially A-form. An analysis of all noncanonical U-U pairs in the context of CUG repeats revealed six different classes of conformations that the noncanonical U-U pairs are able to adopt.

Utilizing the GAAA Tetraloop/Receptor To Facilitate Crystal Packing and Determination of the Structure of a CUG RNA Helix.,Coonrod LA, Lohman JR, Berglund JA Biochemistry. 2012 Oct 12. PMID:23025897<ref>PMID:23025897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 4fnj" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Berglund, J A]]

[[Category: Coonrod, L A]]

[[Category: Lohman, J R]]

[[Category: Cug repeat gaaa tetraloop/receptor]]

[[Category: Rna]]

[[Category: Toxic rna]]