4lw0

<StructureSection load='4lw0' size='340' side='right' caption='[[4lw0]], [[Resolution|resolution]] 1.89&Aring;' scene=''>

<table><tr><td colspan='2'>[[4lw0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LW0 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lvz|4lvz]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lw0 OCA], [http://pdbe.org/4lw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lw0 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The tetrahydrofolate (THF) riboswitch regulates folate transport and metabolism in a number of Firmicutes by cooperatively binding two molecules of THF. To further understand this riboswitch's specificity for THF, binding and regulatory activity of a series of THF analogs and antifolates were examined. Our data reveal that although binding is dominated by the RNA's interactions with the pterin moiety, the para-aminobenzoic acid (pABA) moiety plays a significant role in transcriptional regulation. Further, we find that adenine and several other analogs bind with high affinity by an alternative binding mechanism. Despite a similar affinity to THF, adenine is a poor regulator of transcriptional attenuation. These results demonstrate that binding alone does not determine a compound's effectiveness in regulating the activity of the riboswitch-a complication in current efforts to develop antimicrobials that target these RNAs.

A Disconnect between High-Affinity Binding and Efficient Regulation by Antifolates and Purines in the Tetrahydrofolate Riboswitch.,Trausch JJ, Batey RT Chem Biol. 2014 Feb 20;21(2):205-16. doi: 10.1016/j.chembiol.2013.11.012. Epub, 2014 Jan 2. PMID:24388757<ref>PMID:24388757</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 4lw0" style="background-color:#fffaf0;"></div>

*[[Riboswitch|Riboswitch]]

[[Category: Batey, R T]]

[[Category: Secondary]]

[[Category: Streptococcus mutan]]

[[Category: Terminator region]]

[[Category: Tetrahydrofolate]]

[[Category: Transcription]]