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| | ==Crystal Structure of FVO strain Plasmodium falciparum AMA1== | | ==Crystal Structure of FVO strain Plasmodium falciparum AMA1== |
| - | <StructureSection load='4r1a' size='340' side='right' caption='[[4r1a]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4r1a' size='340' side='right'caption='[[4r1a]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4r1a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4r1a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_FCR-3/Gambia Plasmodium falciparum FCR-3/Gambia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R1A FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4r19|4r19]], [[4r1b|4r1b]], [[4r1c|4r1c]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1a OCA], [http://pdbe.org/4r1a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r1a RCSB], [http://www.ebi.ac.uk/pdbsum/4r1a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1a ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1a OCA], [https://pdbe.org/4r1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r1a RCSB], [https://www.ebi.ac.uk/pdbsum/4r1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AMA1_PLAFG AMA1_PLAFG]] Involved in parasite invasion of erythrocytes. | + | [https://www.uniprot.org/uniprot/AMA1_PLAFG AMA1_PLAFG] Involved in parasite invasion of erythrocytes. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lim, S S]] | + | [[Category: Large Structures]] |
| - | [[Category: McGowan, S]] | + | [[Category: Plasmodium falciparum FCR-3/Gambia]] |
| - | [[Category: Norton, R S]] | + | [[Category: Lim SS]] |
| - | [[Category: Cell invasion]] | + | [[Category: McGowan S]] |
| - | [[Category: Erythrocyte invasion by merozoite]] | + | [[Category: Norton RS]] |
| - | [[Category: Moving junction complex]]
| + | |
| - | [[Category: Pan fold]]
| + | |
| - | [[Category: Ron2]]
| + | |
| Structural highlights
Function
AMA1_PLAFG Involved in parasite invasion of erythrocytes.
Publication Abstract from PubMed
Apical membrane antigen 1 (AMA1) interacts with RON2 to form a protein complex that plays a key role in the invasion of host cells by malaria parasites. Blocking this protein-protein interaction represents a potential route to controlling malaria and related parasitic diseases, but the polymorphic nature of AMA1 has proven to be a major challenge to vaccine-induced antibodies and peptide inhibitors exerting strain-transcending inhibitory effects. Here we present the X-ray crystal structure of AMA1 domains I and II from Plasmodium falciparum strain FVO. We compare our new structure to those of AMA1 from P. falciparum 3D7 and Plasmodium vivax. A combination of normalized B factor analysis and computational methods has been used to investigate the flexibility of the domain I loops and how this correlates with their roles in determining the strain specificity of human antibody responses and inhibitory peptides. We also investigated the domain II loop, a key region involved in inhibitor binding, by comparison of multiple AMA1 crystal structures. Collectively, these results provide valuable insights that should contribute to the design of strain-transcending agents targeting P. falciparum AMA1.
Structure and dynamics of apical membrane antigen 1 from Plasmodium falciparum FVO.,Lim SS, Yang W, Krishnarjuna B, Kannan Sivaraman K, Chandrashekaran IR, Kass I, MacRaild CA, Devine SM, Debono CO, Anders RF, Scanlon MJ, Scammells PJ, Norton RS, McGowan S Biochemistry. 2014 Nov 25;53(46):7310-20. doi: 10.1021/bi5012089. Epub 2014 Nov, 14. PMID:25360546[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lim SS, Yang W, Krishnarjuna B, Kannan Sivaraman K, Chandrashekaran IR, Kass I, MacRaild CA, Devine SM, Debono CO, Anders RF, Scanlon MJ, Scammells PJ, Norton RS, McGowan S. Structure and dynamics of apical membrane antigen 1 from Plasmodium falciparum FVO. Biochemistry. 2014 Nov 25;53(46):7310-20. doi: 10.1021/bi5012089. Epub 2014 Nov, 14. PMID:25360546 doi:http://dx.doi.org/10.1021/bi5012089
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