4agi

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.

Structural highlights

4agi is a 4 chain structure with sequence from Aspergillus fumigatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LECF_ASPFU Multispecific lectin that is able to recognize L-fucose in all possible linkages (PubMed:23695231, PubMed:27058347, PubMed:24340081, PubMed:25760594). These could be found not only in decomposed plant matter in soil, which is the natural environment for A.fumigatus, but also in various epitopes on human tissues (PubMed:25760594). Mediates binding of A.fumigatus conidia to airway mucinin a fucose dependent manner (PubMed:27058347). Stimulates IL-8 production by human bronchial cells in a dose-dependent manner, contributing to the inflammatory response observed upon the exposure of a patient to A.fumigatus, and thus might be an important virulence factor involved in an early stage of A.fumigatus infection (PubMed:24340081).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Aspergillus fumigatus is an important allergen and opportunistic pathogen. Similarly to many other pathogens, it is able to produce lectins that may be involved in the host-pathogen interaction. We focused on the lectin AFL, which was prepared in recombinant form and characterized. Its binding properties were studied using hemagglutination and glycan array analysis. We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including alpha1-6 linked core-fucose, which is an important marker for cancerogenesis. Other biologically relevant saccharides such as sialic acid, d-mannose or d-galactose were not bound. Blood group epitopes of the ABH and Lewis systems were recognized, Le(Y) being the preferred ligand among others. To provide a correlation between the observed functional characteristics and structural basis, AFL was crystallized in a complex with methyl-alpha,l-selenofucoside and its structure was solved using the SAD method. Six binding sites, each with different compositions, were identified per monomer and significant differences from the homologous AAL lectin were found. Structure-derived peptides were utilized to prepare anti-AFL polyclonal antibodies, which suggested the presence of AFL on the Aspergillus' conidia, confirming its expression in vivo. Stimulation of human bronchial cells by AFL led to IL-8 production in a dose-dependent manner. AFL thus probably contributes to the inflammatory response observed upon the exposure of a patient to A. fumigatus. The combination of affinity to human epithelial epitopes, production by conidia and pro-inflammatory activity is remarkable and shows that AFL might be an important virulence factor involved in an early stage of A. fumigatus infection.

A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity.,Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr SC, Lahmann M, Balloy V, Fahy JV, Chignard M, Imberty A, Wimmerova M PLoS One. 2013 Dec 10;8(12):e83077. doi: 10.1371/journal.pone.0083077. PMID:24340081^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kuboi S, Ishimaru T, Tamada S, Bernard EM, Perlin DS, Armstrong D. Molecular characterization of AfuFleA, an L-fucose-specific lectin from Aspergillus fumigatus. J Infect Chemother. 2013 Dec;19(6):1021-8. doi: 10.1007/s10156-013-0614-9. Epub, 2013 May 22. PMID:23695231 doi:http://dx.doi.org/10.1007/s10156-013-0614-9
↑ Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr SC, Lahmann M, Balloy V, Fahy JV, Chignard M, Imberty A, Wimmerova M. A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity. PLoS One. 2013 Dec 10;8(12):e83077. doi: 10.1371/journal.pone.0083077. PMID:24340081 doi:http://dx.doi.org/10.1371/journal.pone.0083077
↑ Houser J, Komarek J, Cioci G, Varrot A, Imberty A, Wimmerova M. Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent. Acta Crystallogr D Biol Crystallogr. 2015 Mar 1;71(Pt 3):442-53. doi:, 10.1107/S1399004714026595. Epub 2015 Feb 26. PMID:25760594 doi:http://dx.doi.org/10.1107/S1399004714026595
↑ Kerr SC, Fischer GJ, Sinha M, McCabe O, Palmer JM, Choera T, Lim FY, Wimmerova M, Carrington SD, Yuan S, Lowell CA, Oscarson S, Keller NP, Fahy JV. FleA Expression in Aspergillus fumigatus Is Recognized by Fucosylated Structures on Mucins and Macrophages to Prevent Lung Infection. PLoS Pathog. 2016 Apr 8;12(4):e1005555. doi: 10.1371/journal.ppat.1005555., eCollection 2016 Apr. PMID:27058347 doi:http://dx.doi.org/10.1371/journal.ppat.1005555
↑ Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr SC, Lahmann M, Balloy V, Fahy JV, Chignard M, Imberty A, Wimmerova M. A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity. PLoS One. 2013 Dec 10;8(12):e83077. doi: 10.1371/journal.pone.0083077. PMID:24340081 doi:http://dx.doi.org/10.1371/journal.pone.0083077

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4agi"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.==
-<StructureSection load='4agi' size='340' side='right' caption='[[4agi]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='4agi' size='340' side='right'caption='[[4agi]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4agi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AGI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4agi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AGI FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SFU:METHYL+6-DEOXY-1-SELENO-ALPHA-L-GALACTOPYRANOSIDE'>SFU</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSK:S-(METHYLSELANYL)-L-CYSTEINE'>CSK</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSK:S-(METHYLSELANYL)-L-CYSTEINE'>CSK</scene>, <scene name='pdbligand=SFU:METHYL+6-DEOXY-1-SELENO-ALPHA-L-GALACTOPYRANOSIDE'>SFU</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4agt|4agt]], [[4ah4|4ah4]], [[4ah5|4ah5]], [[4aha|4aha]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4agi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4agi OCA], [https://pdbe.org/4agi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4agi RCSB], [https://www.ebi.ac.uk/pdbsum/4agi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4agi ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4agi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4agi OCA], [http://pdbe.org/4agi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4agi RCSB], [http://www.ebi.ac.uk/pdbsum/4agi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4agi ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LECF_ASPFU LECF_ASPFU] Multispecific lectin that is able to recognize L-fucose in all possible linkages (PubMed:23695231, PubMed:27058347, PubMed:24340081, PubMed:25760594). These could be found not only in decomposed plant matter in soil, which is the natural environment for A.fumigatus, but also in various epitopes on human tissues (PubMed:25760594). Mediates binding of A.fumigatus conidia to airway mucinin a fucose dependent manner (PubMed:27058347). Stimulates IL-8 production by human bronchial cells in a dose-dependent manner, contributing to the inflammatory response observed upon the exposure of a patient to A.fumigatus, and thus might be an important virulence factor involved in an early stage of A.fumigatus infection (PubMed:24340081).<ref>PMID:23695231</ref> <ref>PMID:24340081</ref> <ref>PMID:25760594</ref> <ref>PMID:27058347</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Cioci, G]]
+[[Category: Aspergillus fumigatus]]
-[[Category: Houser, J]]
+[[Category: Large Structures]]
-[[Category: Imberty, A]]
+[[Category: Cioci G]]
-[[Category: Komarek, J]]
+[[Category: Houser J]]
-[[Category: Kostlanova, N]]
+[[Category: Imberty A]]
-[[Category: Lahmann, M]]
+[[Category: Komarek J]]
-[[Category: Varrot, A]]
+[[Category: Kostlanova N]]
-[[Category: Wimmerova, M]]
+[[Category: Lahmann M]]
-[[Category: Sugar-binding protein]]
+[[Category: Varrot A]]
+[[Category: Wimmerova M]]

4agi

From Proteopedia

Current revision

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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