2uwi

From Proteopedia

(Difference between revisions)

Current revision

Structure of CrmE, a poxvirus TNF receptor

Structural highlights

2uwi is a 2 chain structure with sequence from Vaccinia virus Lister. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Vaccinia virus (VACV), the smallpox vaccine, encodes many proteins that subvert the host immune response. One of these, cytokine response modifier E (CrmE), is secreted by infected cells and protects these cells from apoptotic challenge by tumour necrosis factor alpha (TNFalpha). We have expressed recombinant CrmE from VACV strain Lister in Escherichia coli, shown that the purified protein is monomeric in solution and competent to bind TNFalpha, and solved the structure to 2.0 A resolution. This is the first structure of a virus-encoded tumour necrosis factor receptor (TNFR). CrmE shares significant sequence similarity with mammalian type 2 TNF receptors (TNFSFR1B, p75; TNFR type 2). The structure confirms that CrmE adopts the canonical TNFR fold but only one of the two "ligand-binding" loops of TNFRSF1A is conserved in CrmE, suggesting a mechanism for the higher affinity of poxvirus TNFRs for TNFalpha over lymphotoxin-alpha. The roles of dimerisation and pre-ligand-assembly domains (PLADs) in poxvirus and mammalian TNFR activity are discussed.

Structure of CrmE, a virus-encoded tumour necrosis factor receptor.,Graham SC, Bahar MW, Abrescia NG, Smith GL, Stuart DI, Grimes JM J Mol Biol. 2007 Sep 21;372(3):660-71. Epub 2007 Jul 3. PMID:17681535^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Graham SC, Bahar MW, Abrescia NG, Smith GL, Stuart DI, Grimes JM. Structure of CrmE, a virus-encoded tumour necrosis factor receptor. J Mol Biol. 2007 Sep 21;372(3):660-71. Epub 2007 Jul 3. PMID:17681535 doi:10.1016/j.jmb.2007.06.082

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uwi"

@@ Line 1: / Line 1: @@
-==STRUCTURE OF CRME, A POXVIRUS TNF RECEPTOR==
+==Structure of CrmE, a poxvirus TNF receptor==
-<StructureSection load='2uwi' size='340' side='right' caption='[[2uwi]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='2uwi' size='340' side='right'caption='[[2uwi]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2uwi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccl Vaccl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UWI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2uwi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_Lister Vaccinia virus Lister]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UWI FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwi OCA], [http://pdbe.org/2uwi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uwi RCSB], [http://www.ebi.ac.uk/pdbsum/2uwi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwi ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwi OCA], [https://pdbe.org/2uwi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uwi RCSB], [https://www.ebi.ac.uk/pdbsum/2uwi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwi ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 10: / Line 11: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/2uwi_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/2uwi_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 29: / Line 30: @@
 __TOC__
 </StructureSection>
-[[Category: Vaccl]]
+[[Category: Large Structures]]
-[[Category: Abrescia, N G]]
+[[Category: Vaccinia virus Lister]]
-[[Category: Bahar, M W]]
+[[Category: Abrescia NG]]
-[[Category: Graham, S C]]
+[[Category: Bahar MW]]
-[[Category: Grimes, J M]]
+[[Category: Graham SC]]
-[[Category: Smith, G L]]
+[[Category: Grimes JM]]
-[[Category: Stuart, D I]]
+[[Category: Smith GL]]
-[[Category: Poxvirus tnf receptor]]
+[[Category: Stuart DI]]
-[[Category: Receptor]]
-[[Category: Receptor immunomodulator]]
-[[Category: Tnf alpha receptor]]

2uwi

From Proteopedia

Current revision

Structure of CrmE, a poxvirus TNF receptor

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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