4omh

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==Crystal structure of the bacterial diterpene cyclase COTB2 variant F149L==
==Crystal structure of the bacterial diterpene cyclase COTB2 variant F149L==
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<StructureSection load='4omh' size='340' side='right' caption='[[4omh]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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<StructureSection load='4omh' size='340' side='right'caption='[[4omh]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4omh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OMH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OMH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4omh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_melanosporofaciens Streptomyces melanosporofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OMH FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4omg|4omg]], [[4omi|4omi]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4omh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4omh OCA], [http://pdbe.org/4omh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4omh RCSB], [http://www.ebi.ac.uk/pdbsum/4omh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4omh ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4omh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4omh OCA], [https://pdbe.org/4omh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4omh RCSB], [https://www.ebi.ac.uk/pdbsum/4omh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4omh ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/COTB2_STRMJ COTB2_STRMJ] Catalyzes the cyclization of the linear isoprenoid intermediate geranylgeranyl diphosphate to tricycclic cyclooctat-9-en-7-ol in the cyclooctatin biosynthesis pathway. Cyclooctatin is a potent inhibitor of lysophospholipase.<ref>PMID:19635410</ref> <ref>PMID:24914964</ref>
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Sesquiterpenes and diterpenes are a diverse class of secondary metabolites that are predominantly derived from plants and some prokaryotes. The properties of these natural products encompass antitumor, antibiotic and even insecticidal activities. Therefore, they are interesting commercial targets for the chemical and pharmaceutical industries. Owing to their structural complexity, these compounds are more efficiently accessed by metabolic engineering of microbial systems than by chemical synthesis. This work presents the first crystal structure of a bacterial diterpene cyclase, CotB2 from the soil bacterium Streptomyces melanosporofaciens, at 1.64 A resolution. CotB2 is a diterpene cyclase that catalyzes the cyclization of the linear geranylgeranyl diphosphate to the tricyclic cyclooctat-9-en-7-ol. The subsequent oxidation of cyclooctat-9-en-7-ol by two cytochrome P450 monooxygenases leads to bioactive cyclooctatin. Plasticity residues that decorate the active site of CotB2 have been mutated, resulting in alternative monocyclic, dicyclic and tricyclic compounds that show bioactivity. These new compounds shed new light on diterpene cyclase reaction mechanisms. Furthermore, the product of mutant CotB2(W288G) produced the new antibiotic compound (1R,3E,7E,11S,12S)-3,7,18-dolabellatriene, which acts specifically against multidrug-resistant Staphylococcus aureus. This opens a sustainable route for the industrial-scale production of this bioactive compound.
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The first structure of a bacterial diterpene cyclase: CotB2.,Janke R, Gorner C, Hirte M, Bruck T, Loll B Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1528-37. doi:, 10.1107/S1399004714005513. Epub 2014 May 23. PMID:24914964<ref>PMID:24914964</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4omh" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Brueck, T]]
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[[Category: Large Structures]]
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[[Category: Goerner, C]]
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[[Category: Streptomyces melanosporofaciens]]
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[[Category: Hirte, M]]
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[[Category: Brueck T]]
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[[Category: Janke, R]]
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[[Category: Goerner C]]
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[[Category: Loll, B]]
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[[Category: Hirte M]]
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[[Category: Class i terpene cyclase fold]]
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[[Category: Janke R]]
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[[Category: Geranylgeranyl diphophate]]
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[[Category: Loll B]]
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[[Category: Lyase]]
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[[Category: Terpene cyclase]]
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Current revision

Crystal structure of the bacterial diterpene cyclase COTB2 variant F149L

PDB ID 4omh

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