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| | ==Molecular mechanism for self-protection against type VI secretion system in Vibrio cholerae== | | ==Molecular mechanism for self-protection against type VI secretion system in Vibrio cholerae== |
| - | <StructureSection load='4noo' size='340' side='right' caption='[[4noo]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4noo' size='340' side='right'caption='[[4noo]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4noo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NOO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4noo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NOO FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VC_A0123 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH]), VC_A0124 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4noo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4noo OCA], [https://pdbe.org/4noo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4noo RCSB], [https://www.ebi.ac.uk/pdbsum/4noo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4noo ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4noo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4noo OCA], [http://pdbe.org/4noo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4noo RCSB], [http://www.ebi.ac.uk/pdbsum/4noo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4noo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/VGRG3_VIBCH VGRG3_VIBCH] Part of the type VI secretion system specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:23362380, PubMed:23341465). Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (PubMed:23362380). Additionally, acts directly as an effector and targets the cell wall peptidoglycan layer of prey cells for degradation via its C-terminus (PubMed:23362380, PubMed:23341465). Toxicity is counteracted by a cognate immunity protein TsiV3 (PubMed:23362380, PubMed:24699653, PubMed:23341465).<ref>PMID:23341465</ref> <ref>PMID:23362380</ref> <ref>PMID:24699653</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibch]] | + | [[Category: Large Structures]] |
| - | [[Category: Fan, Z]] | + | [[Category: Vibrio cholerae O1 biovar El Tor str. N16961]] |
| - | [[Category: Jiang, T]] | + | [[Category: Fan Z]] |
| - | [[Category: Xu, M]] | + | [[Category: Jiang T]] |
| - | [[Category: Yang, X]] | + | [[Category: Xu M]] |
| - | [[Category: Chitosanase-fold]]
| + | [[Category: Yang X]] |
| - | [[Category: Glycoside hydrolase effector]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunity protein]]
| + | |
| - | [[Category: Self-protection]]
| + | |
| - | [[Category: Three-helical bundle]]
| + | |
| Structural highlights
Function
VGRG3_VIBCH Part of the type VI secretion system specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:23362380, PubMed:23341465). Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (PubMed:23362380). Additionally, acts directly as an effector and targets the cell wall peptidoglycan layer of prey cells for degradation via its C-terminus (PubMed:23362380, PubMed:23341465). Toxicity is counteracted by a cognate immunity protein TsiV3 (PubMed:23362380, PubMed:24699653, PubMed:23341465).[1] [2] [3]
Publication Abstract from PubMed
VgrG proteins form the spike of the type VI secretion system (T6SS) syringe-like complex. VgrG3 of Vibrio cholerae degrades the peptidoglycan cell wall of rival bacteria via its C-terminal region (VgrG3C) through its muramidase activity. VgrG3C consists of a peptidoglycan-binding domain (VgrG3C(PGB)) and a putative catalytic domain (VgrG3C(CD)), and its activity can be inhibited by its immunity protein partner TsiV3. Here, the crystal structure of V. cholerae VgrG3C(CD) in complex with TsiV3 is presented at 2.3 A resolution. VgrG3C(CD) adopts a chitosanase fold. A dimer of TsiV3 is bound in the deep active-site groove of VgrG3C(CD), occluding substrate binding and distorting the conformation of the catalytic dyad. Gln91 and Arg92 of TsiV3 are located in the centre of the interface and are important for recognition of VgrG3C. Mutation of these residues destabilized the complex and abolished the inhibitory activity of TsiV3 against VgrG3C toxicity in cells. Disruption of TsiV3 dimerization also weakened the complex and impaired the inhibitory activity. These structural, biochemical and functional data define the molecular mechanism underlying the self-protection of V. cholerae and expand the understanding of the role of T6SS in bacterial competition.
Molecular mechanism for self-protection against the type VI secretion system in Vibrio cholerae.,Yang X, Xu M, Wang Y, Xia P, Wang S, Ye B, Tong L, Jiang T, Fan Z Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):1094-103. doi:, 10.1107/S1399004714001242. Epub 2014 Mar 20. PMID:24699653[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brooks TM, Unterweger D, Bachmann V, Kostiuk B, Pukatzki S. Lytic activity of the Vibrio cholerae type VI secretion toxin VgrG-3 is inhibited by the antitoxin TsaB. J Biol Chem. 2013 Mar 15;288(11):7618-7625. doi: 10.1074/jbc.M112.436725. Epub , 2013 Jan 22. PMID:23341465 doi:http://dx.doi.org/10.1074/jbc.M112.436725
- ↑ Dong TG, Ho BT, Yoder-Himes DR, Mekalanos JJ. Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A. 2013 Feb 12;110(7):2623-8. doi: , 10.1073/pnas.1222783110. Epub 2013 Jan 29. PMID:23362380 doi:http://dx.doi.org/10.1073/pnas.1222783110
- ↑ Yang X, Xu M, Wang Y, Xia P, Wang S, Ye B, Tong L, Jiang T, Fan Z. Molecular mechanism for self-protection against the type VI secretion system in Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):1094-103. doi:, 10.1107/S1399004714001242. Epub 2014 Mar 20. PMID:24699653 doi:http://dx.doi.org/10.1107/S1399004714001242
- ↑ Yang X, Xu M, Wang Y, Xia P, Wang S, Ye B, Tong L, Jiang T, Fan Z. Molecular mechanism for self-protection against the type VI secretion system in Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):1094-103. doi:, 10.1107/S1399004714001242. Epub 2014 Mar 20. PMID:24699653 doi:http://dx.doi.org/10.1107/S1399004714001242
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