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| | ==Crystal structure of the DNA mimic protein DMP19== | | ==Crystal structure of the DNA mimic protein DMP19== |
| - | <StructureSection load='3vjz' size='340' side='right' caption='[[3vjz]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3vjz' size='340' side='right'caption='[[3vjz]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vjz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimb Neimb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VJZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vjz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VJZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vkq|3vkq]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NMB0541 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=122586 NEIMB])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vjz OCA], [https://pdbe.org/3vjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vjz RCSB], [https://www.ebi.ac.uk/pdbsum/3vjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vjz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vjz OCA], [http://pdbe.org/3vjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vjz RCSB], [http://www.ebi.ac.uk/pdbsum/3vjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vjz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q9K0P4_NEIMB Q9K0P4_NEIMB] |
| - | DNA mimic proteins occupy the DNA binding sites of DNA-binding proteins, and prevent these sites from being accessed by DNA. We show here that the Neisseria conserved hypothetical protein DMP19 acts as a DNA mimic. The crystal structure of DMP19 shows a dsDNA-like negative charge distribution on the surface, suggesting that this protein should be added to the short list of known DNA mimic proteins. The crystal structure of another related protein, NHTF (Neisseria hypothetical transcription factor), provides evidence that it is a member of the xenobiotic-response element (XRE) family of transcriptional factors. NHTF binds to a palindromic DNA sequence containing a 5'-TGTNAN(11)TNACA-3' recognition box that controls the expression of an NHTF-related operon in which the conserved nitrogen-response protein [i.e. (Protein-PII) uridylyltransferase] is encoded. The complementary surface charges between DMP19 and NHTF suggest specific charge-charge interaction. In a DNA-binding assay, we found that DMP19 can prevent NHTF from binding to its DNA-binding sites. Finally, we used an in situ gene regulation assay to provide evidence that NHTF is a repressor of its down-stream genes and that DMP19 can neutralize this effect. We therefore conclude that the interaction of DMP19 and NHTF provides a novel gene regulation mechanism in Neisseria spps.
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| - | Neisseria conserved protein DMP19 is a DNA mimic protein that prevents DNA binding to a hypothetical nitrogen-response transcription factor.,Wang HC, Ko TP, Wu ML, Ku SC, Wu HJ, Wang AH Nucleic Acids Res. 2012 Feb 28. PMID:22373915<ref>PMID:22373915</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3vjz" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Neimb]] | + | [[Category: Large Structures]] |
| - | [[Category: Ko, T P]] | + | [[Category: Neisseria meningitidis MC58]] |
| - | [[Category: Wang, A H.J]] | + | [[Category: Ko T-P]] |
| - | [[Category: Wang, H C]] | + | [[Category: Wang AH-J]] |
| - | [[Category: Dna mimic]]
| + | [[Category: Wang H-C]] |
| - | [[Category: Gene regulation]]
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| - | [[Category: Helix bundle]]
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