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| | ==H27-14 TCR specific for HLA-A24-Nef134-10== | | ==H27-14 TCR specific for HLA-A24-Nef134-10== |
| - | <StructureSection load='3vxq' size='340' side='right' caption='[[3vxq]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3vxq' size='340' side='right'caption='[[3vxq]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VXQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VXQ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vxm|3vxm]], [[3vxn|3vxn]], [[3vxo|3vxo]], [[3vxp|3vxp]], [[3vxr|3vxr]], [[3vxs|3vxs]], [[3vxt|3vxt]], [[3vxu|3vxu]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxq OCA], [http://pdbe.org/3vxq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vxq RCSB], [http://www.ebi.ac.uk/pdbsum/3vxq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxq OCA], [https://pdbe.org/3vxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vxq RCSB], [https://www.ebi.ac.uk/pdbsum/3vxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Fukai, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Iwamoto, A]] | + | [[Category: Fukai S]] |
| - | [[Category: Shimizu, A]] | + | [[Category: Iwamoto A]] |
| - | [[Category: Yamagata, A]] | + | [[Category: Shimizu A]] |
| - | [[Category: Hiv-1]] | + | [[Category: Yamagata A]] |
| - | [[Category: Immune response]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunogloburin domain]]
| + | |
| - | [[Category: Nef]]
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| - | [[Category: T cell receptor]]
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| - | [[Category: Tcr]]
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| Structural highlights
Publication Abstract from PubMed
We investigated the crystal structure of an HLA-A*2402-restricted CTL epitope in the HIV-1 nef gene (Nef134-10) before (pHLA) or after TCR docking. The wild type epitope and two escape mutants were included in the study. Y135F was an early-appearing major mutation, while F139L was a late-appearing mutation which was selected in the patients without Y135F. F139 was an eminent feature of the Nef134-10 epitope. Wild type-specific TCR was less fit to F139L mutant suggesting that F139L is an escape from the CTL against the wild type epitope. Although Y135F mutation disrupted the hydrogen bond to HLA-A*2402 His70, newly formed hydrogen bond between T138 and His70 kept the conformation of the epitope in the reconstituted pMHC. TCR from Y135F- or dually-specific CTL had unique mode of binding to the mutant epitope. Y135F has been reported as a processing mutant but CTL carrying structurally adequate TCR can be found in the patients.
Structure of TCR and antigen complexes at an immunodominant CTL epitope in HIV-1 infection.,Shimizu A, Kawana-Tachikawa A, Yamagata A, Han C, Zhu D, Sato Y, Nakamura H, Koibuchi T, Carlson J, Martin E, Brumme CJ, Shi Y, Gao GF, Brumme ZL, Fukai S, Iwamoto A Sci Rep. 2013 Nov 6;3:3097. doi: 10.1038/srep03097. PMID:24192765[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shimizu A, Kawana-Tachikawa A, Yamagata A, Han C, Zhu D, Sato Y, Nakamura H, Koibuchi T, Carlson J, Martin E, Brumme CJ, Shi Y, Gao GF, Brumme ZL, Fukai S, Iwamoto A. Structure of TCR and antigen complexes at an immunodominant CTL epitope in HIV-1 infection. Sci Rep. 2013 Nov 6;3:3097. doi: 10.1038/srep03097. PMID:24192765 doi:http://dx.doi.org/10.1038/srep03097
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