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| | ==Crystal structure of rabbit mAb R20 Fab== | | ==Crystal structure of rabbit mAb R20 Fab== |
| - | <StructureSection load='4jo4' size='340' side='right' caption='[[4jo4]], [[Resolution|resolution]] 2.27Å' scene=''> | + | <StructureSection load='4jo4' size='340' side='right'caption='[[4jo4]], [[Resolution|resolution]] 2.27Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4jo4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JO4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JO4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4jo4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JO4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jo1|4jo1]], [[4jo2|4jo2]], [[4jo3|4jo3]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jo4 OCA], [http://pdbe.org/4jo4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jo4 RCSB], [http://www.ebi.ac.uk/pdbsum/4jo4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jo4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jo4 OCA], [https://pdbe.org/4jo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jo4 RCSB], [https://www.ebi.ac.uk/pdbsum/4jo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jo4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[3D structures of monoclonal antibody|3D structures of monoclonal antibody]] | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: European rabbit]] | + | [[Category: Large Structures]] |
| - | [[Category: Kong, X P]] | + | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Pan, R M]] | + | [[Category: Kong XP]] |
| - | [[Category: Antibody]] | + | [[Category: Pan RM]] |
| - | [[Category: Ig]]
| + | |
| - | [[Category: Immune system]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The Rabbit is a commonly used animal model in studying antibody responses in HIV/AIDS vaccine development. However, no rabbit monoclonal antibodies have been developed previously to study the epitope specific antibody responses against HIV-1 envelope glycoproteins (Env), and little is known about how the rabbit immune system can mimic the human immune system in eliciting such antibodies. Here we present structural analyses of two rabbit monoclonal antibodies (mAbs), R56 and R20, against the third variable region (V3) of HIV-1 gp120. R56 recognizes the well-studied immunogenic region in the V3 crown, while R20 targets a less-studied region at the C-terminus of V3. By comparison of the Fab/epitope complex structures of these two antibodies raised by immunization with that of the corresponding human antibodies derived from patients chronically infected by HIV-1, we found that rabbit antibodies can recognize immunogenic regions of gp120 and mimic the binding modes of human antibodies. This result can provide new insight in using the rabbit as an animal model in AIDS vaccine development.
Rabbit anti-HIV-1 monoclonal antibodies raised by immunization can mimic the antigen-binding modes of antibodies derived from HIV-1 infected humans.,Pan R, Sampson JM, Chen Y, Vaine M, Wang S, Lu S, Kong XP J Virol. 2013 Jul 17. PMID:23864637[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pan R, Sampson JM, Chen Y, Vaine M, Wang S, Lu S, Kong XP. Rabbit anti-HIV-1 monoclonal antibodies raised by immunization can mimic the antigen-binding modes of antibodies derived from HIV-1 infected humans. J Virol. 2013 Jul 17. PMID:23864637 doi:10.1128/JVI.00843-13
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