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| | ==Structure of 4C1 Fab in C2221 space group== | | ==Structure of 4C1 Fab in C2221 space group== |
| - | <StructureSection load='3rvu' size='340' side='right' caption='[[3rvu]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3rvu' size='340' side='right'caption='[[3rvu]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rvu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RVU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rvu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RVU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rvt|3rvt]], [[3rvv|3rvv]], [[3rvw|3rvw]], [[3rvx|3rvx]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gm16939 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rvu OCA], [https://pdbe.org/3rvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rvu RCSB], [https://www.ebi.ac.uk/pdbsum/3rvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rvu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rvu OCA], [http://pdbe.org/3rvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rvu RCSB], [http://www.ebi.ac.uk/pdbsum/3rvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rvu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Chapman, M D]] | + | [[Category: Mus musculus]] |
| - | [[Category: Chruszcz, M]] | + | [[Category: Chapman MD]] |
| - | [[Category: Minor, W]] | + | [[Category: Chruszcz M]] |
| - | [[Category: Pomes, A]] | + | [[Category: Minor W]] |
| - | [[Category: Vailes, L D]] | + | [[Category: Pomes A]] |
| - | [[Category: Antibody]]
| + | [[Category: Vailes LD]] |
| - | [[Category: Der f 1]]
| + | |
| - | [[Category: Der p 1]]
| + | |
| - | [[Category: Igg]]
| + | |
| - | [[Category: Immune system]]
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| Structural highlights
Publication Abstract from PubMed
House dust mites produce potent allergens, Der p 1 and Der f 1, that cause allergic sensitization and asthma. Der p 1 and Der f 1 are cysteine proteases that elicit IgE responses in 80% of mite-allergic subjects and have proinflammatory properties. Their antigenic structure is unknown. Here, we present crystal structures of natural Der p 1 and Der f 1 in complex with a monoclonal antibody, 4C1, which binds to a unique cross-reactive epitope on both allergens associated with IgE recognition. The 4C1 epitope is formed by almost identical amino acid sequences and contact residues. Mutations of the contact residues abrogate mAb 4C1 binding and reduce IgE antibody binding. These surface-exposed residues are molecular targets that can be exploited for development of recombinant allergen vaccines.
Molecular determinants for antibody binding on group 1 house dust mite allergens.,Chruszcz M, Pomes A, Glesner J, Vailes LD, Osinski T, Porebski PJ, Majorek KA, Heymann PW, Platts-Mills TA, Minor W, Chapman MD J Biol Chem. 2012 Mar 2;287(10):7388-98. Epub 2011 Dec 30. PMID:22210776[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chruszcz M, Pomes A, Glesner J, Vailes LD, Osinski T, Porebski PJ, Majorek KA, Heymann PW, Platts-Mills TA, Minor W, Chapman MD. Molecular determinants for antibody binding on group 1 house dust mite allergens. J Biol Chem. 2012 Mar 2;287(10):7388-98. Epub 2011 Dec 30. PMID:22210776 doi:10.1074/jbc.M111.311159
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