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Phosphocarrier protein
From Proteopedia
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| - | <StructureSection load=' | + | <StructureSection load='' size='350' side='right' caption='Structure of phosphocarrier protein HPr (PDB entry [[1fu0]])' scene='57/570591/Cv/1'> |
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== Function == | == Function == | ||
'''Phosphocarrier protein HPr''' (HPr) is a component of the sugar phosphotransferase system. The system catalyses the phosphorylation of sugars during their translocation across cell membrane. A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr<ref>PMID:8246840</ref>. A conserved histidine in the N terminal of HPr is the acceptor of the phosphate. In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated. | '''Phosphocarrier protein HPr''' (HPr) is a component of the sugar phosphotransferase system. The system catalyses the phosphorylation of sugars during their translocation across cell membrane. A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr<ref>PMID:8246840</ref>. A conserved histidine in the N terminal of HPr is the acceptor of the phosphate. In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated. | ||
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| + | '''Phosphocarrier protein NPr''' (NPr) is a component of the sugar phosphotransferase system. NPr is a phosphoryl transfer protein, homologous to HPr which functions to regulate nitrogen metabolism<ref>PMID:18421563</ref>. | ||
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| + | '''Phosphocarrier protein III Glc''' (PcpIII) is a signal-transducing component of the phosphoenolpyruvate:glycose phosphotransferase system of ''E. coli''<ref>PMID:11168402</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
| - | The <scene name='57/570591/Cv/ | + | The <scene name='57/570591/Cv/3'>phosphorylation of Ser46</scene> prohibits the HPr to act as a phosphocarrier in the EI reaction<ref>PMID:11054290</ref>. |
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==3D structures of phosphocarrier protein HPr== | ==3D structures of phosphocarrier protein HPr== | ||
| + | [[Phosphocarrier protein HPr 3D structures]] | ||
| - | + | </StructureSection> | |
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| - | **[[2jel]] - EcHPr + antibody <br /> | ||
| - | **[[3eze]], [[3ezb]], [[3eza]] - EcHPr + EI N terminal + PO3 – NMR <br /> | ||
| - | **[[2xdf]] - EcHPr + EI N terminal – NMR <br /> | ||
| - | **[[1ggr]], [[1j6t]], [[1vrc]] - EcHPr + EII N terminal + PO3 – NMR <br /> | ||
| - | **[[2lrk]], [[2lrl]] - EcHPr + EII N terminal – NMR <br /> | ||
| - | **[[1kkl]] - BsHPr (mutant) + HPrK <br /> | ||
| - | **[[1kkm]] - BsHPr (mutant) with phosphoserine + HPrK/P <br /> | ||
| - | **[[2fep]] - BsHPr with phosphoserine + BsCcpA <br /> | ||
| - | **[[1rzr]] – BmHPr with phosphoserine + BmCcpA + DNA - ''Bacillus megaterium''<br /> | ||
| - | **[[2oen]] - BmHPr with phosphoserine + BmCcpA<br /> | ||
| - | **[[2nzu]] - BmHPr with phosphoserine + BmCcpA + glucose-6-phosphate <br /> | ||
| - | **[[2nzv]] - BmHPr with phosphoserine + BmCcpA + fructose-1,6-diphosphate <br /> | ||
| - | **[[3oqm]], [[3oqn]], [[3oqo]] - BsHPr with phosphoserine + BsCcpA + DNA<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
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References
- ↑ Postma PW, Lengeler JW, Jacobson GR. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev. 1993 Sep;57(3):543-94. PMID:8246840
- ↑ Li X, Peterkofsky A, Wang G. Solution structure of NPr, a bacterial signal-transducing protein that controls the phosphorylation state of the potassium transporter-regulating protein IIA Ntr. Amino Acids. 2008 Oct;35(3):531-9. doi: 10.1007/s00726-008-0079-9. Epub 2008 Apr , 18. PMID:18421563 doi:http://dx.doi.org/10.1007/s00726-008-0079-9
- ↑ Maurer T, Doker R, Gorler A, Hengstenberg W, Kalbitzer HR. Three-dimensional structure of the histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis in solution. Eur J Biochem. 2001 Feb;268(3):635-44. PMID:11168402
- ↑ Audette GF, Engelmann R, Hengstenberg W, Deutscher J, Hayakawa K, Quail JW, Delbaere LT. The 1.9 A resolution structure of phospho-serine 46 HPr from Enterococcus faecalis. J Mol Biol. 2000 Nov 3;303(4):545-53. PMID:11054290 doi:10.1006/jmbi.2000.4166
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