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5dnv

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==Crystal structure of KAI2-like protein from Striga (apo state 2)==
==Crystal structure of KAI2-like protein from Striga (apo state 2)==
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<StructureSection load='5dnv' size='340' side='right' caption='[[5dnv]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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<StructureSection load='5dnv' size='340' side='right'caption='[[5dnv]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5dnv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DNV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5dnv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Striga_hermonthica Striga hermonthica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DNV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dnu|5dnu]], [[5dnw|5dnw]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dnv OCA], [http://pdbe.org/5dnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dnv RCSB], [http://www.ebi.ac.uk/pdbsum/5dnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dnv ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dnv OCA], [https://pdbe.org/5dnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dnv RCSB], [https://www.ebi.ac.uk/pdbsum/5dnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dnv ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0M3PN85_STRHE A0A0M3PN85_STRHE]
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The perception of two plant germination inducers, karrikins and strigolactones, are mediated by the proteins KAI2 and D14. Recently, KAI2-type proteins from parasitic weeds, which are possibly related to seed germination induced by strigolactone, have been classified into three clades characterized by different responses to karrikin/strigolactone. Here we characterized a karrikin-binding protein in Striga (ShKAI2iB) that belongs to intermediate-evolving KAI2 and provided the structural bases for its karrikin-binding specificity. Binding assays showed that ShKAI2iB bound karrikins but not strigolactone, differing from other KAI2 and D14. The crystal structures of ShKAI2iB and ShKAI2iB-karrikin complex revealed obvious structural differences in a helix located at the entry of its ligand-binding cavity. This results in a smaller closed pocket, which is also the major cause of ShKAI2iB's specificity of binding karrikin. Our structural study also revealed that a few non-conserved amino acids led to the distinct ligand-binding profile of ShKAI2iB, suggesting that the evolution of KAI2 resulted in its diverse functions.
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Structural basis of unique ligand specificity of KAI2-like protein from parasitic weed Striga hermonthica.,Xu Y, Miyakawa T, Nakamura H, Nakamura A, Imamura Y, Asami T, Tanokura M Sci Rep. 2016 Aug 10;6:31386. doi: 10.1038/srep31386. PMID:27507097<ref>PMID:27507097</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5dnv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Miyakawa, T]]
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[[Category: Large Structures]]
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[[Category: Nakamura, A]]
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[[Category: Striga hermonthica]]
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[[Category: Tanokura, M]]
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[[Category: Miyakawa T]]
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[[Category: Xu, Y]]
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[[Category: Nakamura A]]
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[[Category: Hydrolase]]
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[[Category: Tanokura M]]
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[[Category: Kai2 karrikin striga]]
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[[Category: Xu Y]]

Current revision

Crystal structure of KAI2-like protein from Striga (apo state 2)

PDB ID 5dnv

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