5ik7

From Proteopedia

(Difference between revisions)

Current revision

Laminin A2LG45 I-form, Apo.

Structural highlights

5ik7 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

LAMA2_MOUSE Note=Defects in Lama2 are a cause of murine muscular dystrophy (dy2J).

Function

LAMA2_MOUSE Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Publication Abstract from PubMed

Dystroglycan is a highly glycosylated extracellular matrix receptor with essential functions in skeletal muscle and the nervous system. Reduced matrix binding by alpha-dystroglycan (alpha-DG) due to perturbed glycosylation is a pathological feature of several forms of muscular dystrophy. Like-acetylglucosaminyltransferase (LARGE) synthesizes the matrix-binding heteropolysaccharide [-glucuronic acid-beta1,3-xylose-alpha1,3-]n. Using a dual exoglycosidase digestion, we confirm that this polysaccharide is present on native alpha-DG from skeletal muscle. The atomic details of matrix binding were revealed by a high-resolution crystal structure of laminin-G-like (LG) domains 4 and 5 (LG4 and LG5) of laminin-alpha2 bound to a LARGE-synthesized oligosaccharide. A single glucuronic acid-beta1,3-xylose disaccharide repeat straddles a Ca2+ ion in the LG4 domain, with oxygen atoms from both sugars replacing Ca2+-bound water molecules. The chelating binding mode accounts for the high affinity of this protein-carbohydrate interaction. These results reveal a previously uncharacterized mechanism of carbohydrate recognition and provide a structural framework for elucidating the mechanisms underlying muscular dystrophy.

Structural basis of laminin binding to the LARGE glycans on dystroglycan.,Briggs DC, Yoshida-Moriguchi T, Zheng T, Venzke D, Anderson ME, Strazzulli A, Moracci M, Yu L, Hohenester E, Campbell KP Nat Chem Biol. 2016 Aug 15. doi: 10.1038/nchembio.2146. PMID:27526028^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Briggs DC, Yoshida-Moriguchi T, Zheng T, Venzke D, Anderson ME, Strazzulli A, Moracci M, Yu L, Hohenester E, Campbell KP. Structural basis of laminin binding to the LARGE glycans on dystroglycan. Nat Chem Biol. 2016 Aug 15. doi: 10.1038/nchembio.2146. PMID:27526028 doi:http://dx.doi.org/10.1038/nchembio.2146

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ik7"

Categories: Large Structures | Mus musculus | Briggs DC | Campbell KP | Hohenester E

@@ Line 1: / Line 1: @@
 ==Laminin A2LG45 I-form, Apo.==
-<StructureSection load='5ik7' size='340' side='right' caption='[[5ik7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='5ik7' size='340' side='right'caption='[[5ik7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ik7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IK7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ik7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IK7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ik4|5ik4]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ik7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ik7 OCA], [http://pdbe.org/5ik7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ik7 RCSB], [http://www.ebi.ac.uk/pdbsum/5ik7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ik7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ik7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ik7 OCA], [https://pdbe.org/5ik7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ik7 RCSB], [https://www.ebi.ac.uk/pdbsum/5ik7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ik7 ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/LAMA2_MOUSE LAMA2_MOUSE]] Note=Defects in Lama2 are a cause of murine muscular dystrophy (dy2J).
+[https://www.uniprot.org/uniprot/LAMA2_MOUSE LAMA2_MOUSE] Note=Defects in Lama2 are a cause of murine muscular dystrophy (dy2J).
 == Function ==
-[[http://www.uniprot.org/uniprot/LAMA2_MOUSE LAMA2_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
+[https://www.uniprot.org/uniprot/LAMA2_MOUSE LAMA2_MOUSE] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 </div>
 <div class="pdbe-citations 5ik7" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Laminin|Laminin]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Briggs, D C]]
+[[Category: Large Structures]]
-[[Category: Campbell, K P]]
+[[Category: Mus musculus]]
-[[Category: Hohenester, E]]
+[[Category: Briggs DC]]
-[[Category: Extracellular matrix]]
+[[Category: Campbell KP]]
-[[Category: Lg domain]]
+[[Category: Hohenester E]]
-[[Category: Ligand binding]]
-[[Category: Structural protein]]

5ik7

From Proteopedia

Current revision

Laminin A2LG45 I-form, Apo.

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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