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5gq0

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Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana

Structural highlights

5gq0 is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.31Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ESP_ARATH Converts glucosinolates both to epithionitriles and to simple nitriles in the presence of myrosinase. Promotes the formation of epithionitriles after hydrolysis of alkenylglucosinolates containing a terminal double bond. Mediates indol-3-acetonitrile (IACN) production from indol-3-ylmethyl glucosinolate. Acts as a negative regulator of senescence.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

Specifier proteins are important components of the glucosinolate-myrosinase system, which mediate plant defense against herbivory and pathogen attacks. Upon tissue disruption, glucosinolates are hydrolyzed to instable aglucones by myrosinases, and then aglucones will rearrange to form defensive isothiocyanates. Specifier proteins can redirect this reaction to form other products, such as simple nitriles, epithionitriles and organic thiocyanates instead of isothiocyanates based on the side chain structure of glucosinolate and the type of the specifier proteins. Nevertheless, the molecular mechanism underlying the different product spectrums of various specifier proteins was not fully understood. Here in this study, we solved the crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana (AtESP) at 2.3 A resolution. Structural comparisons with the previously solved structure of thiocyanate forming protein, TFP from Thlaspi arvense (TaTFP) reveal that AtESP shows a dimerization pattern different from TaTFP. Moreover, AtESP harbors a slightly larger active site pocket than TaTFP and several residues around the active site are different between the two proteins, which might account for the different product spectrums of the two proteins. Together, our structural study provides important insights into the molecular mechanisms of specifier proteins and shed light on the basis of their different product spectrums.

Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana provides insights into its product specificity.,Zhang W, Wang W, Liu Z, Xie Y, Wang H, Mu Y, Huang Y, Feng Y Biochem Biophys Res Commun. 2016 Sep 16;478(2):746-51. doi:, 10.1016/j.bbrc.2016.08.019. Epub 2016 Aug 4. PMID:27498030^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lambrix V, Reichelt M, Mitchell-Olds T, Kliebenstein DJ, Gershenzon J. The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia ni herbivory. Plant Cell. 2001 Dec;13(12):2793-807. PMID:11752388
↑ Zabala Mde T, Grant M, Bones AM, Bennett R, Lim YS, Kissen R, Rossiter JT. Characterisation of recombinant epithiospecifier protein and its over-expression in Arabidopsis thaliana. Phytochemistry. 2005 Apr;66(8):859-67. PMID:15845404 doi:http://dx.doi.org/S0031-9422(05)00090-7
↑ Burow M, Muller R, Gershenzon J, Wittstock U. Altered glucosinolate hydrolysis in genetically engineered Arabidopsis thaliana and its influence on the larval development of Spodoptera littoralis. J Chem Ecol. 2006 Nov;32(11):2333-49. PMID:17061170 doi:http://dx.doi.org/10.1007/s10886-006-9149-1
↑ Miao Y, Zentgraf U. The antagonist function of Arabidopsis WRKY53 and ESR/ESP in leaf senescence is modulated by the jasmonic and salicylic acid equilibrium. Plant Cell. 2007 Mar;19(3):819-30. Epub 2007 Mar 16. PMID:17369373 doi:http://dx.doi.org/tpc.106.042705
↑ Burow M, Zhang ZY, Ober JA, Lambrix VM, Wittstock U, Gershenzon J, Kliebenstein DJ. ESP and ESM1 mediate indol-3-acetonitrile production from indol-3-ylmethyl glucosinolate in Arabidopsis. Phytochemistry. 2008 Feb;69(3):663-71. Epub 2007 Oct 24. PMID:17920088 doi:http://dx.doi.org/S0031-9422(07)00534-1
↑ Zhang W, Wang W, Liu Z, Xie Y, Wang H, Mu Y, Huang Y, Feng Y. Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana provides insights into its product specificity. Biochem Biophys Res Commun. 2016 Sep 16;478(2):746-51. doi:, 10.1016/j.bbrc.2016.08.019. Epub 2016 Aug 4. PMID:27498030 doi:http://dx.doi.org/10.1016/j.bbrc.2016.08.019

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5gq0"

Categories: Arabidopsis thaliana | Large Structures | Feng Y | Zhang W

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana==
-<StructureSection load='5gq0' size='340' side='right' caption='[[5gq0]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
+<StructureSection load='5gq0' size='340' side='right'caption='[[5gq0]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5gq0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GQ0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5gq0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GQ0 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gq0 OCA], [http://pdbe.org/5gq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gq0 RCSB], [http://www.ebi.ac.uk/pdbsum/5gq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gq0 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gq0 OCA], [https://pdbe.org/5gq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gq0 RCSB], [https://www.ebi.ac.uk/pdbsum/5gq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gq0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ESP_ARATH ESP_ARATH]] Converts glucosinolates both to epithionitriles and to simple nitriles in the presence of myrosinase. Promotes the formation of epithionitriles after hydrolysis of alkenylglucosinolates containing a terminal double bond. Mediates indol-3-acetonitrile (IACN) production from indol-3-ylmethyl glucosinolate. Acts as a negative regulator of senescence.<ref>PMID:11752388</ref> <ref>PMID:15845404</ref> <ref>PMID:17061170</ref> <ref>PMID:17369373</ref> <ref>PMID:17920088</ref>
+[https://www.uniprot.org/uniprot/ESP_ARATH ESP_ARATH] Converts glucosinolates both to epithionitriles and to simple nitriles in the presence of myrosinase. Promotes the formation of epithionitriles after hydrolysis of alkenylglucosinolates containing a terminal double bond. Mediates indol-3-acetonitrile (IACN) production from indol-3-ylmethyl glucosinolate. Acts as a negative regulator of senescence.<ref>PMID:11752388</ref> <ref>PMID:15845404</ref> <ref>PMID:17061170</ref> <ref>PMID:17369373</ref> <ref>PMID:17920088</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Feng, Y]]
+[[Category: Arabidopsis thaliana]]
-[[Category: Zhang, W]]
+[[Category: Large Structures]]
-[[Category: Beta propeller]]
+[[Category: Feng Y]]
-[[Category: Beta sheet]]
+[[Category: Zhang W]]
-[[Category: Plant protein]]
-[[Category: Specifier protein]]

5gq0

From Proteopedia

Current revision

Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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