5szk

<StructureSection load='5szk' size='340' side='right' caption='[[5szk]], [[Resolution|resolution]] 2.80&Aring;' scene=''>

<table><tr><td colspan='2'>[[5szk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SZK FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5szk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5szk OCA], [http://pdbe.org/5szk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5szk RCSB], [http://www.ebi.ac.uk/pdbsum/5szk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5szk ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/MICLK_HUMAN MICLK_HUMAN]] May cooperate with MAPK1/ERK2 via an intracellular signal transduction pathway in the morphogenetic development of round spermatids to spermatozoa. [[http://www.uniprot.org/uniprot/RAB1B_HUMAN RAB1B_HUMAN]] Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.<ref>PMID:9437002</ref>

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In their active GTP-bound form, Rab proteins interact with proteins termed effector molecules. In this study we have thoroughly characterised a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. Within our study, we show that these effectors display a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate binding sites. Structural analysis allowed us to explain the specificity towards Rab8 family members and the presence of two similar Rab binding sites that must have evolved via gene duplication. This study is the first to thoroughly characterise a Rab effector protein that contains two separate Rab binding sites within a single domain, allowing Micals and EHBPs to bind two Rabs simultaneously, thus suggesting previously unknown functions of these effector molecules in endosomal trafficking.

 

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== References ==

[[Category: Campos, J]]

[[Category: Friese, T]]

[[Category: Fu, Y]]

[[Category: Gazdag, E M]]

[[Category: Goody, R S]]

[[Category: Itzen, A]]

[[Category: Mueller, M P]]

[[Category: Oprisko, A]]

[[Category: Rai, A]]

[[Category: Rab1b]]