This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5lon
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5lon is ON HOLD until Paper Publication Authors: Charenton, C., Taverniti, V., Gaudon-Plesse, C., Back, R., Seraphin, B., Graille, M. Description: ...) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Structure of /K. lactis/ Dcp1-Dcp2 decapping complex.== | |
| + | <StructureSection load='5lon' size='340' side='right'caption='[[5lon]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5lon]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LON FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lon OCA], [https://pdbe.org/5lon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lon RCSB], [https://www.ebi.ac.uk/pdbsum/5lon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lon ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6CIU1_KLULA Q6CIU1_KLULA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Elimination of the 5' cap of eukaryotic mRNAs, known as decapping, is considered to be a crucial, irreversible and highly regulated step required for the rapid degradation of mRNA by Xrn1, the major cytoplasmic 5'-3' exonuclease. Decapping is accomplished by the recruitment of a protein complex formed by the Dcp2 catalytic subunit and its Dcp1 cofactor. However, this complex has a low intrinsic enzymatic activity and requires several accessory proteins such as the Lsm1-7 complex, Pat1, Edc1-Edc2 and/or Edc3 to be fully active. Here we present the crystal structure of the active form of the yeast Kluyveromyces lactis Dcp1-Dcp2 enzyme bound to its product (m7GDP) and its potent activator Edc3. This structure of the Dcp1-Dcp2 complex bound to a cap analog further explains previously published data on substrate binding and provides hints as to the mechanism of Edc3-mediated Dcp2 activation. | ||
| - | + | Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator.,Charenton C, Taverniti V, Gaudon-Plesse C, Back R, Seraphin B, Graille M Nat Struct Mol Biol. 2016 Oct 3. doi: 10.1038/nsmb.3300. PMID:27694841<ref>PMID:27694841</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5lon" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Kluyveromyces lactis NRRL Y-1140]] |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Back R]] | ||
| + | [[Category: Charenton C]] | ||
| + | [[Category: Gaudon-Plesse C]] | ||
| + | [[Category: Graille M]] | ||
| + | [[Category: Seraphin B]] | ||
| + | [[Category: Taverniti V]] | ||
Current revision
Structure of /K. lactis/ Dcp1-Dcp2 decapping complex.
| |||||||||||
