5sxu

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(New page: '''Unreleased structure''' The entry 5sxu is ON HOLD until Paper Publication Authors: Chen, Q., Kinde, M., Cohen, A., Xu, Y., Tang, P. Description: X-ray structure of 2-bromoethanol bo...)
Current revision (12:50, 4 October 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5sxu is ON HOLD until Paper Publication
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==X-ray structure of 2-bromoethanol bound to a pentameric ligand gated ion channel (ELIC) in a desensitized state==
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<StructureSection load='5sxu' size='340' side='right'caption='[[5sxu]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5sxu]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SXU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CN:3-AMINOPROPANE'>3CN</scene>, <scene name='pdbligand=BRJ:2-BROMOETHANOL'>BRJ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sxu OCA], [https://pdbe.org/5sxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5sxu RCSB], [https://www.ebi.ac.uk/pdbsum/5sxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5sxu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structural basis for alcohol modulation of neuronal pentameric ligand-gated ion channels (pLGICs) remains elusive. We determined an inhibitory mechanism of alcohol on the pLGIC Erwinia chrysanthemi (ELIC) through direct binding to the pore. X-ray structures of ELIC co-crystallized with 2-bromoethanol, in both the absence and presence of agonist, reveal 2-bromoethanol binding in the pore near T237(6') and the extracellular domain (ECD) of each subunit at three different locations. Binding to the ECD does not appear to contribute to the inhibitory action of 2-bromoethanol and ethanol as indicated by the same functional responses of wild-type ELIC and mutants. In contrast, the ELIC-alpha1beta3GABAAR chimera, replacing the ELIC transmembrane domain (TMD) with the TMD of alpha1beta3GABAAR, is potentiated by 2-bromoethanol and ethanol. The results suggest a dominant role of the TMD in modulating alcohol effects. The X-ray structures and functional measurements support a pore-blocking mechanism for inhibitory action of short-chain alcohols.
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Authors: Chen, Q., Kinde, M., Cohen, A., Xu, Y., Tang, P.
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Structural Basis of Alcohol Inhibition of the Pentameric Ligand-Gated Ion Channel ELIC.,Chen Q, Wells MM, Tillman TS, Kinde MN, Cohen A, Xu Y, Tang P Structure. 2017 Jan 3;25(1):180-187. doi: 10.1016/j.str.2016.11.007. Epub 2016, Dec 1. PMID:27916519<ref>PMID:27916519</ref>
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Description: X-ray structure of 2-bromoethanol bound to a pentameric ligand gated ion channel (ELIC) in a desensitized state
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Tang, P]]
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<div class="pdbe-citations 5sxu" style="background-color:#fffaf0;"></div>
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[[Category: Xu, Y]]
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[[Category: Cohen, A]]
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==See Also==
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[[Category: Kinde, M]]
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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[[Category: Chen, Q]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Dickeya dadantii 3937]]
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[[Category: Large Structures]]
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[[Category: Chen Q]]
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[[Category: Cohen A]]
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[[Category: Kinde M]]
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[[Category: Tang P]]
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[[Category: Xu Y]]

Current revision

X-ray structure of 2-bromoethanol bound to a pentameric ligand gated ion channel (ELIC) in a desensitized state

PDB ID 5sxu

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