5t64
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5t64 is ON HOLD Authors: Holden, H.M., Thoden, J.B., DOW, G.T. Description: X-ray structure of the C3-methyltransferase KijD1 from Actinomadura kij...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray structure of the C3-methyltransferase KijD1 from Actinomadura kijaniata in complex with TDP and SAH== | |
| + | <StructureSection load='5t64' size='340' side='right'caption='[[5t64]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5t64]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomadura_kijaniata Actinomadura kijaniata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T64 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t64 OCA], [https://pdbe.org/5t64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t64 RCSB], [https://www.ebi.ac.uk/pdbsum/5t64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t64 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B3TMQ9_ACTKI B3TMQ9_ACTKI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Kijanimicin is an antitumor antibiotic isolated from Actinomadura kijaniata. It is composed of three distinct moieties: a pentacyclic core, a monosaccharide referred to as d-kijanose, and a tetrasaccharide chain composed of l-digitoxose units. d-Kijanose is a highly unusual nitro-containing tetradeoxysugar, which requires at least ten enzymes for its production. Here we describe a structural analysis of one of these enzymes, namely KijD1, which functions as a C-3'-methyltransferase using S-adenosylmethionine as its cofactor. For this investigation, two ternary complexes of KijD1, determined in the presence of S-adenosylhomocysteine (SAH) and dTDP or SAH and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-d-glucose, were solved to 1.7 or 1.6 A resolution, respectively. Unexpectedly, these structures, as well as additional biochemical analyses, demonstrated that the quaternary structure of KijD1 is a dimer. Indeed, this is in sharp contrast to that previously observed for the sugar C-3'-methyltransferase isolated from Micromonospora chalcea. By the judicious use of site-directed mutagenesis, it was possible to convert the dimeric form of KijD1 into a monomeric version. The quaternary structure of KijD1 could not have been deduced based solely on bioinformatics approaches, and thus this investigation highlights the continuing need for experimental validation. | ||
| - | + | Structural studies on KijD1, a sugar C-3'-methyltransferase.,Dow GT, Thoden JB, Holden HM Protein Sci. 2016 Sep 6. doi: 10.1002/pro.3034. PMID:27595766<ref>PMID:27595766</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5t64" style="background-color:#fffaf0;"></div> |
| - | [[Category: Holden | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Actinomadura kijaniata]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: DOW GT]] | ||
| + | [[Category: Holden HM]] | ||
| + | [[Category: Thoden JB]] | ||
Current revision
X-ray structure of the C3-methyltransferase KijD1 from Actinomadura kijaniata in complex with TDP and SAH
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