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| | ==Tubulin-MMAF complex== | | ==Tubulin-MMAF complex== |
| - | <StructureSection load='5j2u' size='340' side='right' caption='[[5j2u]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='5j2u' size='340' side='right'caption='[[5j2u]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J2U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5j2u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J2U FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-METHOXY-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-METHOXY-5-METHYL-4-[METHYL-[(2~{S})-3-METHYL-2-[[(2~{S})-3-METHYL-2-(METHYLAMINO)BUTANOYL]AMINO]BUTANOYL]AMINO]HEPTANOYL]PYRROLIDIN-2-YL]-2-METHYL-PROPANOYL]AMINO]-3-PHENYL-PROPANOIC+ACID'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iyz|5iyz]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3WT:(3R,4S,5S)-3-METHOXY-5-METHYL-4-(METHYLAMINO)HEPTANOIC+ACID'>3WT</scene>, <scene name='pdbligand=3WU:(2R,3R)-3-METHOXY-2-METHYL-3-[(2S)-PYRROLIDIN-2-YL]PROPANOIC+ACID'>3WU</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [http://pdbe.org/5j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [http://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [https://pdbe.org/5j2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [https://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> | + | [https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5j2u" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5j2u" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Stathmin-4 3D structures|Stathmin-4 3D structures]] |
| | + | *[[Tubulin 3D Structures|Tubulin 3D Structures]] |
| | + | *[[Tubulin tyrosine ligase 3D structures|Tubulin tyrosine ligase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| - | [[Category: Bargsten, K]] | + | [[Category: Gallus gallus]] |
| - | [[Category: Doronina, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Prota, A E]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Steinmetz, M O]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Sussman, D]] | + | [[Category: Bargsten K]] |
| - | [[Category: Waight, A B]] | + | [[Category: Doronina S]] |
| - | [[Category: Cell cycle]] | + | [[Category: Prota AE]] |
| - | [[Category: Cytoskeleton]] | + | [[Category: Steinmetz MO]] |
| - | [[Category: Microtubule]] | + | [[Category: Sussman D]] |
| - | [[Category: Tubulin fold]] | + | [[Category: Waight AB]] |
| Structural highlights
5j2u is a 8 chain structure with sequence from Bos taurus, Gallus gallus, Rattus norvegicus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.5Å |
| Ligands: | , , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
TBA1B_BOVIN Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Publication Abstract from PubMed
The auristatin class of microtubule destabilizers are highly potent cytotoxic agents against several cancer cell types when delivered as antibody drug conjugates. Here we describe the high resolution structures of tubulin in complex with both monomethyl auristatin E and F and unambiguously define the trans-configuration of both ligands at the Val-Dil amide bond in their tubulin bound state. Moreover, we illustrate how peptidic vinca-site agents carrying terminal carboxylate residues may exploit an observed extended hydrogen bond network with the M-loop Arg278 to greatly improve the affinity of the corresponding analogs and to maintain the M-loop in an incompatible conformation for productive lateral tubulin-tubulin contacts in microtubules. Our results highlight a potential, previously undescribed molecular mechanism by which peptidic vinca-site agents maintain unparalleled potency as microtubule-destabilizing agents.
Structural Basis of Microtubule Destabilization by Potent Auristatin Anti-Mitotics.,Waight AB, Bargsten K, Doronina S, Steinmetz MO, Sussman D, Prota AE PLoS One. 2016 Aug 12;11(8):e0160890. doi: 10.1371/journal.pone.0160890., eCollection 2016. PMID:27518442[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Waight AB, Bargsten K, Doronina S, Steinmetz MO, Sussman D, Prota AE. Structural Basis of Microtubule Destabilization by Potent Auristatin Anti-Mitotics. PLoS One. 2016 Aug 12;11(8):e0160890. doi: 10.1371/journal.pone.0160890., eCollection 2016. PMID:27518442 doi:http://dx.doi.org/10.1371/journal.pone.0160890
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