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| | ==Crystal structure of mouse CNTN5 Ig1-Ig4 domains== | | ==Crystal structure of mouse CNTN5 Ig1-Ig4 domains== |
| - | <StructureSection load='5e4i' size='340' side='right' caption='[[5e4i]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='5e4i' size='340' side='right'caption='[[5e4i]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5e4i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E4I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5e4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E4I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e4i OCA], [http://pdbe.org/5e4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e4i RCSB], [http://www.ebi.ac.uk/pdbsum/5e4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e4i ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e4i OCA], [https://pdbe.org/5e4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e4i RCSB], [https://www.ebi.ac.uk/pdbsum/5e4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e4i ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CNTN5_MOUSE CNTN5_MOUSE]] Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons (By similarity). Involved in neuronal activity in the auditory system.<ref>PMID:12653969</ref> | + | [https://www.uniprot.org/uniprot/CNTN5_MOUSE CNTN5_MOUSE] Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons (By similarity). Involved in neuronal activity in the auditory system.<ref>PMID:12653969</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bouyain, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Nikolaienko, R M]] | + | [[Category: Mus musculus]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Bouyain S]] |
| - | [[Category: Horseshoe-like conformation]] | + | [[Category: Nikolaienko RM]] |
| - | [[Category: Immunoglobulin-like domain]]
| + | |
| - | [[Category: Neural cell adhesion molecule]]
| + | |
| Structural highlights
Function
CNTN5_MOUSE Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons (By similarity). Involved in neuronal activity in the auditory system.[1]
Publication Abstract from PubMed
Protein tyrosine phosphatase receptor type G (RPTPgamma/PTPRG) interacts in vitro with contactin-3-6 (CNTN3-6), a group of glycosylphosphatidyl-anchored cell adhesion molecules involved in the wiring of the nervous system. In addition to PTPRG, CNTNs associate with multiple transmembrane proteins and signal inside the cell via cis-binding partners to alleviate the absence of an intracellular region. Here, we use comprehensive biochemical and structural analyses to demonstrate that PTPRG[middot]CNTN3-6 complexes share similar binding affinities and a conserved arrangement. Furthermore, as a first step to identifying PTPRG.CNTN complexes in vivo, we found that PTPRG and CNTN3 associate in the outer segments of mouse rod photoreceptor cells. In particular, PTPRG and CNTN3 form cis-complexes at the surface of photoreceptors, yet interact in trans when expressed on the surfaces of apposing cells. Further structural analyses suggest that all CNTN ectodomains adopt a bent conformation and might lie parallel to the cell surface to accommodate these cis and trans binding modes. Taken together, these studies identify a PTPRG.CNTN complex in vivo and provide novel insights into PTPRG- and CNTN- mediated signaling.
Structural Basis for Interactions Between Contactin Family Members and Protein Tyrosine Phosphatase Receptor Type G in Neural Tissues.,Nikolaienko RM, Hammel M, Dubreuil V, Zalmai R, Hall DR, Mehzabeen N, Karuppan SJ, Harroch S, Stella SL, Bouyain S J Biol Chem. 2016 Aug 18. pii: jbc.M116.742163. PMID:27539848[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li H, Takeda Y, Niki H, Ogawa J, Kobayashi S, Kai N, Akasaka K, Asano M, Sudo K, Iwakura Y, Watanabe K. Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2. Eur J Neurosci. 2003 Mar;17(5):929-36. PMID:12653969
- ↑ Nikolaienko RM, Hammel M, Dubreuil V, Zalmai R, Hall DR, Mehzabeen N, Karuppan SJ, Harroch S, Stella SL, Bouyain S. Structural Basis for Interactions Between Contactin Family Members and Protein Tyrosine Phosphatase Receptor Type G in Neural Tissues. J Biol Chem. 2016 Aug 18. pii: jbc.M116.742163. PMID:27539848 doi:http://dx.doi.org/10.1074/jbc.M116.742163
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