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| | ==Crystal structure of mouse CNTN6 FN1-FN3 domains== | | ==Crystal structure of mouse CNTN6 FN1-FN3 domains== |
| - | <StructureSection load='5e55' size='340' side='right' caption='[[5e55]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='5e55' size='340' side='right'caption='[[5e55]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5e55]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E55 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E55 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5e55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E55 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5e4q|5e4q]], [[5e4s|5e4s]], [[5e4i|5e4i]], [[5e52|5e52]], [[5e53|5e53]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.702Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e55 OCA], [http://pdbe.org/5e55 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e55 RCSB], [http://www.ebi.ac.uk/pdbsum/5e55 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e55 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e55 OCA], [https://pdbe.org/5e55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e55 RCSB], [https://www.ebi.ac.uk/pdbsum/5e55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e55 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CNTN6_MOUSE CNTN6_MOUSE]] Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus (By similarity). Involved in motor coordination.<ref>PMID:12884264</ref> | + | [https://www.uniprot.org/uniprot/CNTN6_MOUSE CNTN6_MOUSE] Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus (By similarity). Involved in motor coordination.<ref>PMID:12884264</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bouyain, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Nikolaienko, R M]] | + | [[Category: Mus musculus]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Bouyain S]] |
| - | [[Category: Fibronectin type iii domain]] | + | [[Category: Nikolaienko RM]] |
| - | [[Category: Neural cell adhesion molecule]]
| + | |
| Structural highlights
Function
CNTN6_MOUSE Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus (By similarity). Involved in motor coordination.[1]
Publication Abstract from PubMed
Protein tyrosine phosphatase receptor type G (RPTPgamma/PTPRG) interacts in vitro with contactin-3-6 (CNTN3-6), a group of glycosylphosphatidyl-anchored cell adhesion molecules involved in the wiring of the nervous system. In addition to PTPRG, CNTNs associate with multiple transmembrane proteins and signal inside the cell via cis-binding partners to alleviate the absence of an intracellular region. Here, we use comprehensive biochemical and structural analyses to demonstrate that PTPRG[middot]CNTN3-6 complexes share similar binding affinities and a conserved arrangement. Furthermore, as a first step to identifying PTPRG.CNTN complexes in vivo, we found that PTPRG and CNTN3 associate in the outer segments of mouse rod photoreceptor cells. In particular, PTPRG and CNTN3 form cis-complexes at the surface of photoreceptors, yet interact in trans when expressed on the surfaces of apposing cells. Further structural analyses suggest that all CNTN ectodomains adopt a bent conformation and might lie parallel to the cell surface to accommodate these cis and trans binding modes. Taken together, these studies identify a PTPRG.CNTN complex in vivo and provide novel insights into PTPRG- and CNTN- mediated signaling.
Structural Basis for Interactions Between Contactin Family Members and Protein Tyrosine Phosphatase Receptor Type G in Neural Tissues.,Nikolaienko RM, Hammel M, Dubreuil V, Zalmai R, Hall DR, Mehzabeen N, Karuppan SJ, Harroch S, Stella SL, Bouyain S J Biol Chem. 2016 Aug 18. pii: jbc.M116.742163. PMID:27539848[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Takeda Y, Akasaka K, Lee S, Kobayashi S, Kawano H, Murayama S, Takahashi N, Hashimoto K, Kano M, Asano M, Sudo K, Iwakura Y, Watanabe K. Impaired motor coordination in mice lacking neural recognition molecule NB-3 of the contactin/F3 subgroup. J Neurobiol. 2003 Sep 5;56(3):252-65. PMID:12884264 doi:http://dx.doi.org/10.1002/neu.10222
- ↑ Nikolaienko RM, Hammel M, Dubreuil V, Zalmai R, Hall DR, Mehzabeen N, Karuppan SJ, Harroch S, Stella SL, Bouyain S. Structural Basis for Interactions Between Contactin Family Members and Protein Tyrosine Phosphatase Receptor Type G in Neural Tissues. J Biol Chem. 2016 Aug 18. pii: jbc.M116.742163. PMID:27539848 doi:http://dx.doi.org/10.1074/jbc.M116.742163
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