1oqj

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[[Image:1oqj.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the SAND domain from glucocorticoid modulatory element binding protein-1 (GMEB1)==
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|PDB= 1oqj |SIZE=350|CAPTION= <scene name='initialview01'>1oqj</scene>, resolution 1.55&Aring;
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<StructureSection load='1oqj' size='340' side='right'caption='[[1oqj]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1oqj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OQJ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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|GENE= GMEB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oqj OCA], [https://pdbe.org/1oqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oqj RCSB], [https://www.ebi.ac.uk/pdbsum/1oqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oqj ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1h5p|1H5P]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oqj OCA], [http://www.ebi.ac.uk/pdbsum/1oqj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oqj RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/GMEB1_HUMAN GMEB1_HUMAN] Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses.
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== Evolutionary Conservation ==
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'''Crystal structure of the SAND domain from glucocorticoid modulatory element binding protein-1 (GMEB1)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oq/1oqj_consurf.spt"</scriptWhenChecked>
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The glucocorticoid-modulatory element-binding proteins, GMEB1 and GMEB2, are ubiquitous, multifunctional DNA-binding proteins with important roles in the modulation of transcription upon steroid hormone activation. The GMEB proteins have intrinsic transactivation ability, but also control the glucocorticoid response via direct binding to the glucocorticoid receptor. They are also mandatory host proteins for Parvovirus replication. Here we present the 1.55 A resolution crystal structure of a central portion of GMEB1, encompassing its SAND domain, which shares 80% sequence identity with the GMEB2 SAND domain. We demonstrate that this domain, also present in numerous proteins implicated in chromatin-associated transcriptional regulation, is necessary and sufficient to bind the glucocorticoid-modulatory element (GME) DNA target. We use nuclear magnetic resonance (NMR) and binding studies to map the DNA recognition surface to an alpha-helical region exposing the conserved KDWK motif. Using site-directed mutagenesis, key residues for DNA binding are identified. In contrast to the previously determined NMR structure of the Sp100b SAND domain, we find that the GMEB1 SAND domain also comprises a zinc-binding motif. Although the zinc ion is not necessary for DNA binding, it is found to determine the C-terminal conformation of the GMEB1 SAND domain. We also show that homologous zinc-binding motifs exist in a subset of SAND domain proteins and probe the roles of this novel motif.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1OQJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQJ OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oqj ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure and nuclear magnetic resonance analyses of the SAND domain from glucocorticoid modulatory element binding protein-1 reveals deoxyribonucleic acid and zinc binding regions., Surdo PL, Bottomley MJ, Sattler M, Scheffzek K, Mol Endocrinol. 2003 Jul;17(7):1283-95. Epub 2003 Apr 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12702733 12702733]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bottomley, M J.]]
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[[Category: Bottomley MJ]]
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[[Category: Sattler, M.]]
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[[Category: Sattler M]]
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[[Category: Scheffzek, K.]]
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[[Category: Scheffzek K]]
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[[Category: Surdo, P L.]]
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[[Category: Surdo PL]]
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[[Category: alpha-beta fold]]
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[[Category: kdwk motif]]
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[[Category: sand domain]]
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[[Category: zinc-binding motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:48:54 2008''
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Current revision

Crystal structure of the SAND domain from glucocorticoid modulatory element binding protein-1 (GMEB1)

PDB ID 1oqj

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