4ylp

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{{Large structure}}
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==E. coli Transcription Initiation Complex - 16-bp spacer and 5-nt RNA==
==E. coli Transcription Initiation Complex - 16-bp spacer and 5-nt RNA==
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<StructureSection load='4ylp' size='340' side='right' caption='[[4ylp]], [[Resolution|resolution]] 5.50&Aring;' scene=''>
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<StructureSection load='4ylp' size='340' side='right'caption='[[4ylp]], [[Resolution|resolution]] 5.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ylp]] is a 27 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YLP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ylp]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YLP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.5&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yln|4yln]], [[4ylo|4ylo]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ylp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ylp OCA], [https://pdbe.org/4ylp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ylp RCSB], [https://www.ebi.ac.uk/pdbsum/4ylp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ylp ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ylp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ylp OCA], [http://pdbe.org/4ylp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ylp RCSB], [http://www.ebi.ac.uk/pdbsum/4ylp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ylp ProSAT]</span></td></tr>
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</table>
</table>
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{{Large structure}}
 
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RPOC_ECO24 RPOC_ECO24]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOB_ECO24 RPOB_ECO24]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOA_ECO24 RPOA_ECO24]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOZ_ECO24 RPOZ_ECO24]] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. [[http://www.uniprot.org/uniprot/RPOD_ECOLI RPOD_ECOLI]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
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[https://www.uniprot.org/uniprot/RPOA_ECOLI RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059]
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
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*[[Sigma factor|Sigma factor]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Escherichia coli]]
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[[Category: Steitz, T A]]
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[[Category: Large Structures]]
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[[Category: Zuo, Y]]
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[[Category: Synthetic construct]]
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[[Category: Dna bubble]]
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[[Category: Steitz TA]]
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[[Category: Initiation complex]]
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[[Category: Zuo Y]]
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[[Category: Rna polymerase]]
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[[Category: Transcription-dna-rna complex]]
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Current revision

E. coli Transcription Initiation Complex - 16-bp spacer and 5-nt RNA

PDB ID 4ylp

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