1osl

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[[Image:1osl.gif|left|200px]]
 
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{{Structure
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==Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence==
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|PDB= 1osl |SIZE=350|CAPTION= <scene name='initialview01'>1osl</scene>
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<StructureSection load='1osl' size='340' side='right'caption='[[1osl]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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<table><tr><td colspan='2'>[[1osl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OSL FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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|GENE= LACI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1osl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osl OCA], [https://pdbe.org/1osl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1osl RCSB], [https://www.ebi.ac.uk/pdbsum/1osl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1osl ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1l1m|1L1M]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1osl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osl OCA], [http://www.ebi.ac.uk/pdbsum/1osl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1osl RCSB]</span>
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1osl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1osl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Interaction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site.
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'''Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence'''
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Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes.,Kalodimos CG, Biris N, Bonvin AM, Levandoski MM, Guennuegues M, Boelens R, Kaptein R Science. 2004 Jul 16;305(5682):386-9. PMID:15256668<ref>PMID:15256668</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1osl" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Interaction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site.
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*[[Lac repressor|Lac repressor]]
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== References ==
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==About this Structure==
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<references/>
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1OSL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSL OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes., Kalodimos CG, Biris N, Bonvin AM, Levandoski MM, Guennuegues M, Boelens R, Kaptein R, Science. 2004 Jul 16;305(5682):386-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15256668 15256668]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boelens, R.]]
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[[Category: Boelens R]]
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[[Category: Bonvin, A M.J J.]]
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[[Category: Bonvin AMJJ]]
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[[Category: Kalodimos, C G.]]
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[[Category: Kalodimos CG]]
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[[Category: Kaptein, R.]]
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[[Category: Kaptein R]]
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[[Category: lac repressor]]
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[[Category: nonspecific interaction]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:45 2008''
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Current revision

Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence

PDB ID 1osl

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