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5ja1

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==EntF, a Terminal Nonribosomal Peptide Synthetase Module Bound to the MbtH-Like Protein YbdZ==
==EntF, a Terminal Nonribosomal Peptide Synthetase Module Bound to the MbtH-Like Protein YbdZ==
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<StructureSection load='5ja1' size='340' side='right' caption='[[5ja1]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='5ja1' size='340' side='right'caption='[[5ja1]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ja1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JA1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ja1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JA1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=75C:5-({[(2R,3S)-3-AMINO-4-HYDROXY-2-{[2-({N-[(2R)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-BETA-ALANYL}AMINO)ETHYL]SULFANYL}BUTYL]SULFONYL}AMINO)-5-DEOXYADENOSINE'>75C</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ja1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ja1 OCA], [http://pdbe.org/5ja1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ja1 RCSB], [http://www.ebi.ac.uk/pdbsum/5ja1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ja1 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=75C:5-({[(2R,3S)-3-AMINO-4-HYDROXY-2-{[2-({N-[(2R)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-BETA-ALANYL}AMINO)ETHYL]SULFANYL}BUTYL]SULFONYL}AMINO)-5-DEOXYADENOSINE'>75C</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ja1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ja1 OCA], [https://pdbe.org/5ja1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ja1 RCSB], [https://www.ebi.ac.uk/pdbsum/5ja1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ja1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ENTF_ECOLI ENTF_ECOLI]] Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE). [[http://www.uniprot.org/uniprot/YBDZ_ECOLI YBDZ_ECOLI]] Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. Plays a role in the catalytic function of EntF. It is required for adenylation of amino acids in non-ribosomal peptide biosynthesis.<ref>PMID:20845982</ref>
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[https://www.uniprot.org/uniprot/ENTF_ECOLI ENTF_ECOLI] Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE).
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Gulick, A M]]
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[[Category: Escherichia coli K-12]]
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[[Category: Miller, B R]]
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[[Category: Large Structures]]
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[[Category: Adenylation]]
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[[Category: Gulick AM]]
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[[Category: Biosynthetic protein]]
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[[Category: Miller BR]]
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[[Category: Condensation]]
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[[Category: Ligase]]
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[[Category: Mbth-like protein]]
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[[Category: Nonribosomal peptide synthetase]]
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[[Category: Nrp]]
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[[Category: Pcp]]
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[[Category: Phosphopantetheine]]
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[[Category: Thioesterase]]
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Current revision

EntF, a Terminal Nonribosomal Peptide Synthetase Module Bound to the MbtH-Like Protein YbdZ

PDB ID 5ja1

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