5erg

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==Crystal structure of the two-subunit tRNA m1A58 methyltransferase TRM6-TRM61 in complex with SAM==
==Crystal structure of the two-subunit tRNA m1A58 methyltransferase TRM6-TRM61 in complex with SAM==
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<StructureSection load='5erg' size='340' side='right' caption='[[5erg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='5erg' size='340' side='right'caption='[[5erg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5erg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ERG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5erg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ERG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.202&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eqj|5eqj]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_(adenine(58)-N(1))-methyltransferase tRNA (adenine(58)-N(1))-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.220 2.1.1.220] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5erg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erg OCA], [https://pdbe.org/5erg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5erg RCSB], [https://www.ebi.ac.uk/pdbsum/5erg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5erg ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5erg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erg OCA], [http://pdbe.org/5erg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5erg RCSB], [http://www.ebi.ac.uk/pdbsum/5erg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5erg ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TRM6_YEAST TRM6_YEAST]] Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.<ref>PMID:10779558</ref> <ref>PMID:7542616</ref> <ref>PMID:9851972</ref> [[http://www.uniprot.org/uniprot/TRM61_YEAST TRM61_YEAST]] Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.<ref>PMID:10779558</ref> <ref>PMID:9539420</ref> <ref>PMID:9851972</ref>
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[https://www.uniprot.org/uniprot/TRM61_YEAST TRM61_YEAST] Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.<ref>PMID:10779558</ref> <ref>PMID:9539420</ref> <ref>PMID:9851972</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The N(1) methylation of adenine at position 58 (m(1)A58) of tRNA is an important post-transcriptional modification, which is vital for maintaining the stability of the initiator methionine tRNAi(Met). In eukaryotes, this modification is performed by the TRM6-TRM61 holoenzyme. To understand the molecular mechanism that underlies the cooperation of TRM6 and TRM61 in the methyl transfer reaction, we determined the crystal structure of TRM6-TRM61 holoenzyme from Saccharomyces cerevisiae in the presence and absence of its methyl donor S-Adenosyl-L-methionine (SAM). In the structures, two TRM6-TRM61 heterodimers assemble as a heterotetramer. Both TRM6 and TRM61 subunits comprise an N-terminal beta-barrel domain linked to a C-terminal Rossmann-fold domain. TRM61 functions as the catalytic subunit, containing a methyl donor (SAM) binding pocket. TRM6 diverges from TRM61, lacking the conserved motifs used for binding SAM. However, TRM6 cooperates with TRM61 forming an L-shaped tRNA binding regions. Collectively, our results provide a structural basis for better understanding the m(1)A58 modification of tRNA occurred in Saccharomyces cerevisiae.
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Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 from Saccharomyces cerevisiae.,Wang M, Zhu Y, Wang C, Fan X, Jiang X, Ebrahimi M, Qiao Z, Niu L, Teng M, Li X Sci Rep. 2016 Sep 1;6:32562. doi: 10.1038/srep32562. PMID:27582183<ref>PMID:27582183</ref>
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==See Also==
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*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5erg" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fan, X]]
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[[Category: Large Structures]]
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[[Category: Jiang, X]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Li, X]]
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[[Category: Fan X]]
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[[Category: Teng, M]]
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[[Category: Jiang X]]
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[[Category: Wang, C]]
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[[Category: Li X]]
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[[Category: Wang, M]]
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[[Category: Teng M]]
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[[Category: Zhu, Y]]
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[[Category: Wang C]]
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[[Category: Complex]]
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[[Category: Wang M]]
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[[Category: Methylation]]
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[[Category: Zhu Y]]
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[[Category: Sam]]
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[[Category: Transferase]]
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[[Category: Trna]]
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Current revision

Crystal structure of the two-subunit tRNA m1A58 methyltransferase TRM6-TRM61 in complex with SAM

PDB ID 5erg

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