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| | ==Bifunctional Methionine Sulfoxide Reductase AB (MsrAB) from Treponema denticola== | | ==Bifunctional Methionine Sulfoxide Reductase AB (MsrAB) from Treponema denticola== |
| - | <StructureSection load='5fa9' size='340' side='right' caption='[[5fa9]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='5fa9' size='340' side='right'caption='[[5fa9]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fa9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FA9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fa9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_denticola_ATCC_35405 Treponema denticola ATCC 35405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FA9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=D1D:(4S,5S)-1,2-DITHIANE-4,5-DIOL'>D1D</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.302Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-methionine_(S)-S-oxide_reductase Peptide-methionine (S)-S-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.4.11 1.8.4.11] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D1D:(4S,5S)-1,2-DITHIANE-4,5-DIOL'>D1D</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fa9 OCA], [http://pdbe.org/5fa9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fa9 RCSB], [http://www.ebi.ac.uk/pdbsum/5fa9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fa9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fa9 OCA], [https://pdbe.org/5fa9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fa9 RCSB], [https://www.ebi.ac.uk/pdbsum/5fa9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fa9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/Q73PT7_TREDE Q73PT7_TREDE]] Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.[HAMAP-Rule:MF_01401] | + | [https://www.uniprot.org/uniprot/Q73PT7_TREDE Q73PT7_TREDE] Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.[HAMAP-Rule:MF_01401] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Han, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Hwang, K Y]] | + | [[Category: Treponema denticola ATCC 35405]] |
| - | [[Category: Kim, H Y]] | + | [[Category: Han A]] |
| - | [[Category: Son, J]] | + | [[Category: Hwang KY]] |
| - | [[Category: Fusion protein]] | + | [[Category: Kim H-Y]] |
| - | [[Category: Oxidoreductase]] | + | [[Category: Son J]] |
| - | [[Category: Protein oxidation]]
| + | |
| Structural highlights
Function
Q73PT7_TREDE Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.[HAMAP-Rule:MF_01401]
Publication Abstract from PubMed
Many bacteria, particularly pathogens, possess methionine sulfoxide reductase A (MsrA) and B (MsrB) as a fusion form (MsrAB). However, it is not clear why they possess a fusion MsrAB form rather than the separate enzymes that exist in most organisms. In this study, we performed biochemical and kinetic analyses of MsrAB from Treponema denticola (TdMsrAB), single-domain forms (TdMsrA and TdMsrB), and catalytic Cys mutants (TdMsrAB(C11S) and TdMsrAB(C285S)). We found that the catalytic efficiency of both MsrA and MsrB increased after fusion of the domains and that the linker region (iloop) that connects TdMsrA and TdMsrB is required for the higher catalytic efficiency of TdMsrAB. We also determined the crystal structure of TdMsrAB at 2.3 A, showing that the iloop mainly interacts with TdMsrB via hydrogen bonds. Further kinetic analysis using the iloop mutants revealed that the iloop-TdMsrB interactions are critical to MsrB and MsrA activities. We also report the structure in which an oxidized form of dithiothreitol, an in vitro reductant for MsrA and MsrB, is present in the active site of TdMsrA. Collectively, the results of this study reveal an essential role of the iloop in maintaining the higher catalytic efficiency of the MsrAB fusion enzyme and provide a better understanding of why the MsrAB enzyme exists as a fused form.
Essential Role of the Linker Region in the Higher Catalytic Efficiency of a Bifunctional MsrA-MsrB Fusion Protein.,Han AR, Kim MJ, Kwak GH, Son J, Hwang KY, Kim HY Biochemistry. 2016 Sep 13;55(36):5117-27. doi: 10.1021/acs.biochem.6b00544. Epub , 2016 Sep 1. PMID:27551953[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Han AR, Kim MJ, Kwak GH, Son J, Hwang KY, Kim HY. Essential Role of the Linker Region in the Higher Catalytic Efficiency of a Bifunctional MsrA-MsrB Fusion Protein. Biochemistry. 2016 Sep 13;55(36):5117-27. doi: 10.1021/acs.biochem.6b00544. Epub , 2016 Sep 1. PMID:27551953 doi:http://dx.doi.org/10.1021/acs.biochem.6b00544
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