5ews

<StructureSection load='5ews' size='340' side='right' caption='[[5ews]], [[Resolution|resolution]] 2.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[5ews]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EWS FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ews FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ews OCA], [http://pdbe.org/5ews PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ews RCSB], [http://www.ebi.ac.uk/pdbsum/5ews PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ews ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/LEG2_HUMAN LEG2_HUMAN]] This protein binds beta-galactoside. Its physiological function is not yet known.

Galectin-2 (Gal-2) plays a role in cancer, myocardial infarction, immune response, and gastrointestinal tract diseases. The only reported crystal structure of Gal-2 shows that it is a dimer in which the monomer subunits have almost identical structures, each binding with one molecule of lactose. In this study, we crystallized Gal-2 under new conditions that produced three crystal structures. In each Gal-2 dimer structure, lactose was shown to be bound to only one of the carbohydrate recognition domain subunits. In solution studies, the thermal shift assay demonstrated that inequivalent monomer subunits in the Gal-2 dimer become equivalent upon ligand binding. In addition, galectin-mediated erythrocyte agglutination assays using lactose and larger complex polysaccharides as inhibitors showed the structural differences between Gal-1 and Gal-2. Overall, our results reveal some novel aspects to the structural differentiation in Gal-2 and expand the potential for different types of molecular interactions that may be specific to this lectin.

 

== References ==

[[Category: Si, Y L]]

[[Category: Su, J Y]]

[[Category: Galectin]]

[[Category: Lactose]]

[[Category: Sugar binding protein]]