5ey0
From Proteopedia
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==Crystal structure of CodY from Staphylococcus aureus with GTP and Ile== | ==Crystal structure of CodY from Staphylococcus aureus with GTP and Ile== | ||
| - | <StructureSection load='5ey0' size='340' side='right' caption='[[5ey0]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='5ey0' size='340' side='right'caption='[[5ey0]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5ey0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EY0 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5ey0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu3 Staphylococcus aureus subsp. aureus Mu3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EY0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EY0 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=ILE:ISOLEUCINE'>ILE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ey0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ey0 OCA], [https://pdbe.org/5ey0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ey0 RCSB], [https://www.ebi.ac.uk/pdbsum/5ey0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ey0 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/CODY_STAA8 CODY_STAA8] DNA-binding global transcriptional regulator which is involved in the adaptive response to starvation and acts by directly or indirectly controlling the expression of numerous genes in response to nutrient availability. During rapid exponential growth, CodY is highly active and represses genes whose products allow adaptation to nutrient depletion.[HAMAP-Rule:MF_00621]<ref>PMID:27116338</ref> In S.aureus, targets include over 200 genes (PubMed:20363936, PubMed:27116338). Acts mainly as a repressor of genes involved in amino acid transport and metabolism, including the branched-chain amino acids (BCAAs) biosynthetic operon (PubMed:19251851, PubMed:20363936, PubMed:27116338, PubMed:29357354). Several genes involved in nucleotide synthesis and transport are activated (PubMed:19251851). Binds to a 21-bp conserved DNA motif, the CodY-binding site (PubMed:20363936). Additionally, in pathogenic bacteria, CodY also regulates virulence gene expression and provides a regulatory link between metabolism and pathogenesis (PubMed:18156263, PubMed:19251851, PubMed:20363936, PubMed:27116338, PubMed:29378891). Genes encoding virulence and defense factors are either up- or down-regulated by CodY (PubMed:19251851). Among others, is involved in the repression of the accessory gene regulator (agr), the hemolytic alpha-toxin (hla) gene, and the icaADBC operon, responsible for the production of polysaccharide intercellular adhesin (PIA), a major contributor to biofilm formation in S.aureus (PubMed:18156263, PubMed:19251851). Also regulates the expression of thermonuclease (nuc) via the Sae two-component system, by binding directly to the sae P1 promoter region and blocking the binding of the positive regulator SaeR (PubMed:27116338, PubMed:29378891). It restrains Sae-dependent production of leukocidins (PubMed:29378891). CodY also controls the sae locus indirectly through Agr and Rot-mediated repression of the sae P1 promoter (PubMed:29378891). The virulence genes regulated by CodY fall into three groups: one group is regulated directly by CodY, a second group is indirectly regulated by CodY, in particular through its repression of the agr and sae loci, and a third group is regulated in two ways, by direct repression and by repression via another regulator (PubMed:19251851, PubMed:20363936, PubMed:29378891). S.aureus may use CodY to limit host damage to only the most severe starvation conditions (PubMed:27116338). Modulation of central metabolism, virulence gene expression, and biofilm-associated genes to optimize growth on preferred carbon sources until starvation sets in may require coordinated action of CodY and the carbon catabolite protein A (CcpA), another global transcriptional regulator (PubMed:35735992).<ref>PMID:18156263</ref> <ref>PMID:19251851</ref> <ref>PMID:20363936</ref> <ref>PMID:27116338</ref> <ref>PMID:29357354</ref> <ref>PMID:29378891</ref> <ref>PMID:35735992</ref> |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Staphylococcus aureus subsp. aureus Mu3]] |
| - | [[Category: | + | [[Category: Han A]] |
| - | [[Category: | + | [[Category: Hwang KY]] |
| - | + | ||
Current revision
Crystal structure of CodY from Staphylococcus aureus with GTP and Ile
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