1p0h

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[[Image:1p0h.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Rv0819 from Mycobacterium Tuberculosis MshD-Mycothiol Synthase Coenzyme A Complex==
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|PDB= 1p0h |SIZE=350|CAPTION= <scene name='initialview01'>1p0h</scene>, resolution 1.60&Aring;
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<StructureSection load='1p0h' size='340' side='right'caption='[[1p0h]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>
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<table><tr><td colspan='2'>[[1p0h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P0H FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= Rv0819 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p0h OCA], [https://pdbe.org/1p0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p0h RCSB], [https://www.ebi.ac.uk/pdbsum/1p0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p0h ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ozp|1OZP]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p0h OCA], [http://www.ebi.ac.uk/pdbsum/1p0h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p0h RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MSHD_MYCTU MSHD_MYCTU] Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol.<ref>PMID:12375100</ref>
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== Evolutionary Conservation ==
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'''Crystal Structure of Rv0819 from Mycobacterium Tuberculosis MshD-Mycothiol Synthase Coenzyme A Complex'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/1p0h_consurf.spt"</scriptWhenChecked>
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Mycothiol is the predominant low-molecular weight thiol produced by actinomycetes, including Mycobacterium tuberculosis. The last reaction in the biosynthetic pathway for mycothiol is catalyzed by mycothiol synthase (MshD), which acetylates the cysteinyl amine of cysteine-glucosamine-inositol (Cys-GlcN-Ins). The crystal structure of MshD was determined in the presence of coenzyme A and acetyl-CoA. MshD consists of two tandem-repeated domains, each exhibiting the Gcn5-related N-acetyltransferase (GNAT) fold. These two domains superimpose with a root-mean-square deviation of 1.7 A over 88 residues, and each was found to bind one molecule of coenzyme, although the binding sites are quite different. The C-terminal domain has a similar active site to many GNAT members in which the acetyl group of the coenzyme is presented to an open active site slot. However, acetyl-CoA bound to the N-terminal domain is buried, and is apparently not positioned to promote acetyl transfer. A modeled substrate complex indicates that Cys-GlcN-Ins would only fill a portion of a negatively charged channel located between the two domains. This is the first structure determined for an enzyme involved in the biosynthesis of mycothiol.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1P0H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0H OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p0h ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases., Vetting MW, Roderick SL, Yu M, Blanchard JS, Protein Sci. 2003 Sep;12(9):1954-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12930994 12930994]
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<references/>
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[[Category: Mycobacterium tuberculosis h37rv]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Blanchard, J S.]]
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[[Category: Large Structures]]
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[[Category: Roderick, S L.]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Vetting, M W.]]
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[[Category: Blanchard JS]]
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[[Category: Yu, M.]]
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[[Category: Roderick SL]]
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[[Category: acetyltransferase]]
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[[Category: Vetting MW]]
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[[Category: coenzyme a complex]]
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[[Category: Yu M]]
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[[Category: gnat fold]]
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[[Category: mshd]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:52:59 2008''
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Current revision

Crystal Structure of Rv0819 from Mycobacterium Tuberculosis MshD-Mycothiol Synthase Coenzyme A Complex

PDB ID 1p0h

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