1p15

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[[Image:1p15.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the D2 domain of RPTPa==
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|PDB= 1p15 |SIZE=350|CAPTION= <scene name='initialview01'>1p15</scene>, resolution 2.00&Aring;
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<StructureSection load='1p15' size='340' side='right'caption='[[1p15]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1p15]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P15 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= PTPRA OR LRP OR PTPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p15 OCA], [https://pdbe.org/1p15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p15 RCSB], [https://www.ebi.ac.uk/pdbsum/1p15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p15 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1p13|1P13]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p15 OCA], [http://www.ebi.ac.uk/pdbsum/1p15 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p15 RCSB]</span>
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[https://www.uniprot.org/uniprot/PTPRA_MOUSE PTPRA_MOUSE]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/1p15_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p15 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the D2 domain of RPTPa'''
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==See Also==
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*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The receptor protein tyrosine phosphatase alpha (RPTPalpha) is a transmembrane receptor with two intracellular protein tyrosine phosphatase domains, a catalytically active membrane proximal domain (D1) and a membrane distal phosphatase domain with minimal catalytic activity (D2). Here we elucidate the crystal structure of RPTPalpha's D2 domain. Unlike D1, D2 exists as a monomer and lacks the N-terminal inhibitory wedge motif. The N-terminal portion of D2 is disordered, and this region linking D1 to D2 is proteolytically labile in solution whether part of D2 alone or tethered to D1, indicating that the polypeptide backbone of this part of D2 is highly flexible, and therefore accessible to proteases under native conditions. Furthermore, we have crystallized the SH2 domain of the protein tyrosine kinase c-Src, a RPTPalpha substrate, with a phosphopeptide encompassing the C-terminal phosphorylation site of D2 (pTyr789). The SH2 domain of Src binds RPTPalpha in an extended conformation. The structural and functional data support a D1-D2 arrangement with significant flexibility between phosphatase domains of RPTPalpha that is likely to be important for dynamic alterations in intra- and/or intermolecular interactions that are critical for RPTPalpha function.
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[[Category: Large Structures]]
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==About this Structure==
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1P15 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P15 OCA].
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==Reference==
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The structure of the membrane distal phosphatase domain of RPTPalpha reveals interdomain flexibility and an SH2 domain interaction region., Sonnenburg ED, Bilwes A, Hunter T, Noel JP, Biochemistry. 2003 Jul 8;42(26):7904-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12834342 12834342]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Bilwes A]]
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[[Category: Single protein]]
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[[Category: Hunter T]]
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[[Category: Bilwes, A.]]
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[[Category: Noel JP]]
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[[Category: Hunter, T.]]
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[[Category: Sonnenburg ED]]
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[[Category: Noel, J P.]]
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[[Category: Sonnenburg, E D.]]
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[[Category: hydrolase]]
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[[Category: phosphorylation]]
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[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:53:17 2008''
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Current revision

Crystal structure of the D2 domain of RPTPa

PDB ID 1p15

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